Cyclin D1 overexpression detected by a simple competitive reverse transcription-polymerase chain reaction assay for lymphoid malignancies

Japanese Journal of Cancer Research : Gann
T TaniguchiT Motokura

Abstract

t(11;14)(q13;q32) observed in B-cell malignancies is associated with cyclin D1 (bcl-1, PRAD1, CCND1) overexpression. We devised a simple competitive reverse transcription-polymerase chain reaction (RT-PCR) assay for rapid detection of cyclin D1 overexpression. Sharing a single upstream primer derived from a homologous sequence in cyclins D1, D2 and D3, each PCR product serves as a competitor and cyclin D1 overexpression is determined by comparing the intensities of the three amplified products. We analyzed cyclin D1 in clinical specimens from 104 patients with lymphoid malignancies. Cyclin D1 overexpression was evident in 13 of 104 (7/72 non-Hodgkin's lymphomas, 0/6 adult T-cell lymphoma/leukemias, 0/4 Hodgkin's diseases, 0/11 acute lymphoblastic leukemias, 3/4 multiple myelomas, 1/2 Waldenström's macroglobulinemias, 1/2 prolymphocytic leukemias and 1/3 chronic lymphocytic leukemias). Among 72 patients for whom cytogenetic studies had been done, all 7 patients with t(11;14) were positive. The relative expression levels of D-type cyclins altered dramatically in the presence of t(11;14). Thus, this RT-PCR assay can identify tumors with cyclin D1 overexpression. Cyclin D1 overexpression was frequent in extranodal specimens (11 out...Continue Reading

References

Jul 1, 1992·The American Journal of Surgical Pathology·P M BanksE S Jaffe
Nov 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·C L RosenbergA Arnold
Apr 11, 1991·Nature·T MotokuraA Arnold
Oct 1, 1991·Molecular and Cellular Biology·D A WithersT C Meeker
Oct 1, 1995·Cancer Genetics and Cytogenetics·H KobayashiT Motokura
Nov 18, 1994·Cell·C J Sherr
May 25, 1993·Biochimica Et Biophysica Acta·T Motokura, A Arnold
Jun 18, 1993·Cell·C J Sherr

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Citations

Jun 10, 2000·British Journal of Haematology·W Hoechtlen-VollmarD Seidel
Dec 13, 2005·Carcinogenesis·Jason K SicklickAnna Mae Diehl
Mar 2, 2002·European Journal of Haematology·T RasmussenH E Johnsen

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