PMID: 9422379Jan 9, 1998Paper

Cyclin D2 interacts with cdk-5 and modulates cellular cdk-5/p35 activity

Journal of Neurochemistry
S GuidatoC C Miller

Abstract

Cyclin-dependent kinase-5 (cdk-5) is a serine/threonine kinase that displays neurone-specific activity. Experimental manipulation of cdk-5 expression in neurones has shown that cdk-5 is essential for proper development of the nervous system and, in particular, for outgrowth of neurites. Such observations suggest that cdk-5 activity must be tightly controlled during development of the nervous system. To identify possible regulators of cdk-5, we used the yeast two-hybrid system to search for proteins that interact with cdk-5. In two independent yeast transformation events, cyclin D2 interacted with cdk-5. Immunoprecipitation experiments confirmed that cyclin D2 and cdk-5 interact in mammalian cells. Cyclin D2 did not activate cdk-5 as assayed using three different substrates, which was in contrast to a known cdk-5 activator, p35. However, cyclin D2 expression led to a decrease in cdk-5/p35 activity in transfected cells. As cyclin D2 and cdk-5 are known to share overlapping patterns of expression during development of the CNS, the results presented here suggest a role for cyclin D2 in modulating cdk-5 activity in postmitotic developing neurones.

Citations

Apr 1, 1999·FEBS Letters·M MoorthamerB Chaudhuri
Jun 1, 2005·Journal of Child Neurology·Shigeaki KanataniKazunori Nakajima
Mar 4, 2010·Molecular Reproduction and Development·Jean-Claude LozanoGérard Peaucellier
Feb 6, 1999·The Journal of Biological Chemistry·A I FletcherE L Stuenkel
Oct 31, 2001·The Journal of Biological Chemistry·Pushkar SharmaHarish C Pant
Mar 2, 1999·Biochemical and Biophysical Research Communications·M Moorthamer, B Chaudhuri
Nov 19, 2021·PloS One·Grzegorz A CzapskiJoanna B Strosznajder
May 24, 2011·Mechanisms of Ageing and Development·Jinqiu ZhuZixu Mao
Sep 20, 2011·Cellular Signalling·Erick Contreras-VallejosChristian Gonzalez-Billault

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