Cyclin-dependent kinases govern formation and maintenance of the nucleolus

The Journal of Cell Biology
Valentina SirriPascal Roussel

Abstract

In higher eukaryotic cells, the nucleolus is a nuclear compartment assembled at the beginning of interphase, maintained during interphase, and disorganized during mitosis. Even if its structural organization appears to be undissociable from its function in ribosome biogenesis, the mechanisms that govern the formation and maintenance of the nucleolus are not elucidated. To determine if cell cycle regulators are implicated, we investigated the putative role of the cyclin-dependent kinases (CDKs) on ribosome biogenesis and nucleolar organization. Inhibition of CDK1-cyclin B during mitosis leads to resumption of rDNA transcription, but is not sufficient to induce proper processing of the pre-rRNA and total relocalization of the processing machinery into rDNA transcription sites. Similarly, at the exit from mitosis, both translocation of the late processing machinery and pre-rRNA processing are impaired in a reversible manner by CDK inhibitors. Therefore, CDK activity seems indispensable for the building of functional nucleoli. Furthermore, inhibition of CDKs in interphasic cells also hampered proper pre-rRNA processing and induced a dramatic disorganization of the nucleolus. Thus, we propose that the mechanisms governing both forma...Continue Reading

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Citations

Dec 6, 2005·Histochemistry and Cell Biology·Danièle Hernandez-Verdun
Jul 13, 2006·Histochemistry and Cell Biology·Danièle Hernandez-Verdun
Nov 30, 2007·Histochemistry and Cell Biology·Valentina SirriDanièle Hernandez-Verdun
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Methods Mentioned

BETA
in situ transcription
electron microscopy
FCS
PCR
Assay
electrophoresis

Software Mentioned

NIH Image
Adobe Photoshop

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