Cyclin E expression in operable breast cancer quantified using real-time RT-PCR: a comparative study with immunostaining

Japanese Journal of Clinical Oncology
Piotr PotemskiRadzislaw Kordek

Abstract

The main purpose of this retrospective study was to compare cyclin E expression levels in operable breast cancer patients determined using real-time RT-PCR and immunostaining. The prognostic relevance of cyclin E was also investigated. Specimens of invasive ductal breast cancer tissues obtained from 124 women during radical mastectomy were analyzed. Of the tumor samples, 40.3 and 59.7% showed high expression of cyclin E in RT-PCR and immunostaining, respectively. The overall agreement probability was 0.032 according to Scott's pi statistic. With a median follow-up of 55.5 months, cyclin E expression assessed using immunostaining was an independent negative prognostic factor in the node positive group (hazard ratio 3.1; 95% CI 1.0-9.2; P = 0.045). Cyclin E expression correlated with absence of steroid receptors and younger age. RT-PCR results did not predict survival in any group of patients. Disagreement between real-time RT-PCR and immunostaining was demonstrated. Immunostaining seems to be the more reliable method for assessing cyclin E in breast cancer cells.

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Citations

Jun 19, 2013·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Sheng GaoCheng Lu
Jan 27, 2016·Tumour Biology : the Journal of the International Society for Oncodevelopmental Biology and Medicine·Satu LuhtalaJorma Isola
Dec 24, 2015·Daru : Journal of Faculty of Pharmacy, Tehran University of Medical Sciences·Sureerat BuahormChanpen Chanchao
Jul 8, 2010·Breast Cancer Research and Treatment·Yuehan WuRebecca S Hartley
Dec 3, 2015·Asian Pacific Journal of Cancer Prevention : APJCP·Hamed MontazeriMohammad Hossein Ghahremani

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