PMID: 9192874Jun 1, 1997Paper

Cyclin E-induced S phase without activation of the pRb/E2F pathway

Genes & Development
J LukasJ Bartek

Abstract

In cells of higher eukaryotes, cyclin D-dependent kinases Cdk4 and Cdk6 and, possibly, cyclin E-dependent Cdk2 positively regulate the G1- to S-phase transition, by phosphorylating the retinoblastoma protein (pRb), thereby releasing E2F transcription factors that control S-phase genes. Here we performed microinjection and transfection experiments using rat R12 fibroblasts, their derivatives conditionally overexpressing cyclins D1 or E, and human U-2-OS cells, to explore the action of G1 cyclins and the relationship of E2F and cyclin E in S-phase induction. We demonstrate that ectopic expression of cyclin E, but not cyclin D1, can override G1 arrest imposed by either the p16INK4a Cdk inhibitor specific for Cdk4 and Cdk6 or a novel phosphorylation-deficient mutant pRb. Several complementary approaches to assess E2F activation, including quantitative reporter assays in live cells, showed that the cyclin E-induced S phase and completion of the cell division cycle can occur in the absence of E2F-mediated transactivation. Together with the ability of cyclin E to overcome a G1 block induced by expression of dominant-negative mutant DP-1, a heterodimeric partner of E2Fs, these results provide evidence for a cyclin E-controlled S phase-...Continue Reading

References

Apr 1, 1992·Current Opinion in Cell Biology·L F Johnson
Jul 3, 1995·Proceedings of the National Academy of Sciences of the United States of America·R H MedemaR A Weinberg
Jul 18, 1995·Proceedings of the National Academy of Sciences of the United States of America·M F RousselC J Sherr
May 1, 1995·Molecular and Cellular Biology·M OhtsuboM Pagano
May 15, 1995·Genes & Development·C J Sherr, J M Roberts
Jul 1, 1995·Molecular and Cellular Biology·D Resnitzky, S I Reed
Jun 1, 1995·Genes & Development·R L BeijersbergenR Bernards
Mar 9, 1995·Nature·D O Morgan
Jan 1, 1994·Advances in Cancer Research·J Y WangP J Welch
Sep 15, 1994·Nature·G Peters
Dec 24, 1993·Science·S van den Heuvel, E Harlow
Jun 18, 1993·Cell·C J Sherr
Dec 19, 1995·Proceedings of the National Academy of Sciences of the United States of America·K OhtaniJ R Nevins
Apr 16, 1996·Proceedings of the National Academy of Sciences of the United States of America·M A IkedaJ R Nevins
Apr 5, 1996·The Journal of Biological Chemistry·E S Knudsen, J Y Wang
Apr 1, 1996·Molecular and Cellular Biology·K MobergJ A Lees
Jul 1, 1996·Molecular and Cellular Biology·J BotzP Jansen-Dürr
Jul 1, 1996·Molecular and Cellular Biology·C L WuE Harlow
Apr 1, 1996·Current Biology : CB·D J Mann, N C Jones
Feb 1, 1996·Current Opinion in Genetics & Development·J W Harper, S J Elledge
Oct 1, 1996·Genes & Development·R J DuronioP H O'Farrell
Nov 1, 1996·Molecular and Cellular Biology·H Hirai, C J Sherr
Dec 1, 1996·Current Opinion in Cell Biology·J BartekJ Lukas

❮ Previous
Next ❯

Citations

Apr 13, 1999·Journal of Cellular Physiology·P StieglerA Giordano
Oct 12, 1999·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·W G Kaelin
Jun 18, 2002·Journal of Cellular Physiology·Yael Leshem, Orna Halevy
Sep 25, 2007·Bulletin of Mathematical Biology·M N ObeyesekereG Auchmuty
Feb 4, 2006·Journal of Cancer Research and Clinical Oncology·In Kyoung Lim
May 17, 2005·Gynecologic Oncology·Synne KnoppRuth Holm
May 30, 2001·Molecular and Cellular Endocrinology·N Takuwa, Y Takuwa
Mar 16, 2001·Biochimica Et Biophysica Acta·P D Adams
Jan 13, 1998·Current Opinion in Cell Biology·M D Planas-Silva, R A Weinberg
Apr 8, 1998·Current Opinion in Genetics & Development·S Mittnacht
Aug 8, 1998·Trends in Cell Biology·C BouchardM Eilers
Oct 27, 1998·Trends in Biochemical Sciences·J O Funk, D A Galloway
Oct 13, 2000·Molecular Cell·J L BruceN Dyson
Apr 20, 2001·Cytokine & Growth Factor Reviews·D JoyceR G Pestell
Jan 8, 1999·Mutation Research·M HengstschlägerE Hengstschläger-Ottnad
Apr 15, 2000·Cell Research·D Donjerkovic, D W Scott
Sep 25, 2008·Proceedings of the National Academy of Sciences of the United States of America·Masahiro ShudaYuan Chang
Jun 16, 2009·Proceedings of the National Academy of Sciences of the United States of America·Jan J MolenaarHuib N Caron
Oct 19, 2011·The Journal of Biological Chemistry·Matthew T BlahnaJoseph P Mizgerd
Mar 23, 2000·The Journal of Experimental Medicine·R HalabanJ Germino
Mar 26, 2003·Genes & Development·Maxim V FrolovNicholas J Dyson
Apr 16, 1998·Genes & Development·B R CalviA C Spradling
Apr 10, 1999·Genes & Development·M KretzschmarJ Massagué
Sep 18, 2001·Journal of Virology·D ChattopadhyayM L Harter
May 22, 2002·Journal of Virology·Frederick A Dick, Nicholas J Dyson
Dec 14, 1999·Molecular and Cellular Biology·F ZindyM F Roussel
Apr 25, 2000·Molecular and Cellular Biology·E Santoni-RugiuJ Lukas

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cell Checkpoints & Regulators

Cell cycle checkpoints are a series of complex checkpoint mechanisms that detect DNA abnormalities and ensure that DNA replication and repair are complete before cell division. They are primarily regulated by cyclins, cyclin-dependent kinases, and the anaphase-promoting complex/cyclosome. Here is the latest research.