Cyclin E overexpression responsible for growth of human hepatic tumors with p21WAF1/CIP1/SDI1

Biochemical and Biophysical Research Communications
T TsujiM Namba

Abstract

We examined a relationship between p21WAF1/CIP1/SDI1 and cell-cycle-related proteins in 12 human liver tumor cell lines (JHH-1, -2, -4, -5, -6, -7; HLE; HuH-7; Hep3B; PLC/PRF/5; HuH-6; HepG2). Seven (JHH-1, -2, -5, -6, -7; Hep3B; HepG2) out of eight cell lines having p21WAF1/CIP1/SDI1 protein overexpressed cyclin E protein, although one of them (JHH-5) overexpressed a reduced size of cyclin E. The rest (HuH-6) of the 8 cell lines with p21WAF1/CIP1/SDI1 showed a decreased expression of cyclin E. Four cell lines (JHH-4; HLE; HuH-7; PLC/PRF/5) deficient of p21WAF1/CIP1/SDI1 protein did not overexpress cyclin E protein. As to expression of the other cell-cycle-related proteins, cyclin A, cyclin D1, CDK2 or CDK4, no significant difference was detected among the 12 cell lines. These findings indicate that the human liver tumor cell lines which have the p21WAF1/CIP1/SDI1-inducible barriers of the cell cycle progression can go through the G1/S checkpoint by overexpressing cyclin E.

Citations

Dec 29, 2010·Proceedings of the National Academy of Sciences of the United States of America·Chiara BraconiTushar Patel
Feb 6, 2003·International Journal of Cancer. Journal International Du Cancer·Fredrik ErlandssonAnders Zetterberg
May 15, 2009·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Marco BreinigMichael André Kern
Oct 28, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Benjamin GoeppertMichael André Kern

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