PMID: 2116420Aug 1, 1990Paper

Cycling of intracellular pH during cell division of Xenopus embryos is a cytoplasmic activity depending on protein synthesis and phosphorylation

The Journal of Cell Biology
N Grandin, M Charbonneau

Abstract

In Xenopus embryos, the successive and rapid cell divisions that follow fertilization are accompanied by periodic oscillations of intracellular pH (pHi). Cycling of pHi occurs in phase with several other oscillatory activities, namely nuclear divisions, M phase-promoting factor (MPF) activity, and surface contraction waves (SCWs). We report that treatments that abolish cycling of MPF activity and the SCWs also suppress the pHi oscillations, whereas those that block cell division without affecting neither MPF activity nor the SCWs do not suppress the pHi oscillations. Experiments on enucleated oocytes, matured in vitro and activated, demonstrated that the activity governing the rhythmicity of the pHi oscillations resided in the cytoplasm of the oocyte. In this respect, the activity responsible for the pHi oscillations was different from that which drives the SCWs, which necessitated the presence of the oocyte germinal vesicle (Ohsumi et al., 1986), but more closely resembled MPF activity that did not require the presence of the oocyte germinal vesicle (Dabauvalle et al., 1988). In mature eggs enucleated at the time of egg activation, the pHi oscillations were similar to those in control nucleated eggs, whereas the period between...Continue Reading

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Citations

Jan 1, 1997·Cell Motility and the Cytoskeleton·K WatanabeY Hamaguchi
Jun 1, 1992·Pflügers Archiv : European journal of physiology·H J WestphaleH Oberleithner
Aug 19, 2010·The Journal of Membrane Biology·Matthieu MarinJean-Pierre Vilain
Jul 28, 2001·Development, Growth & Differentiation·S KurodaA Shinagawa
Feb 1, 1991·The Journal of Cell Biology·N Grandin, M Charbonneau
Aug 1, 1993·BioEssays : News and Reviews in Molecular, Cellular and Developmental Biology·J M Baltz
Sep 21, 2019·The Journal of Cell Biology·Enako HosodaKazuyoshi Chiba

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