PMID: 7539916Jun 6, 1995Paper

Cyclins as markers of tumor proliferation: immunocytochemical studies in breast cancer

Proceedings of the National Academy of Sciences of the United States of America
A DuttaS Lester

Abstract

We have developed methods to use anticyclin A, B, and E antibodies as reagents to specifically detect proliferating cells in specific phases of the cell cycle in formalin-fixed, paraffin-embedded sections of tissues and cells. Staining of 48 archival cases of breast cancer showed that these antibodies estimate the tumor proliferation fraction and therefore are potentially useful for the prediction of prognosis. A subset of cancers had a high frequency of tumor cells expressing cyclins A and E, out of proportion to other proliferation markers, suggesting that these tumors may have deregulated cyclin expression. In addition to recognizing authentic cyclin E in the nucleus of proliferating cells, anticyclin E antibody cross-reacted with a cytoplasmic protein in nonproliferating endothelial cells. This cross-reaction allows the simultaneous visualization and quantitation of microvessels in the tumors, measuring a second potential predictor of breast cancer prognosis, tumor angiogenesis.

References

Dec 16, 1992·Journal of the National Cancer Institute·N WeidnerG Gasparini
Aug 6, 1992·The New England Journal of Medicine·J R HarrisW Willett
Jun 25, 1992·The New England Journal of Medicine·W L McGuire, G M Clark
Aug 13, 1992·The New England Journal of Medicine·J R HarrisW Willett
Jan 3, 1991·The New England Journal of Medicine·N WeidnerJ Folkman
Mar 9, 1989·The New England Journal of Medicine·G M ClarkW L McGuire
Sep 30, 1993·International Journal of Cancer. Journal International Du Cancer·M ToiT Tominaga
Feb 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Keyomarsi, A B Pardee
Jun 15, 1993·Cancer·S M VeroneseP Lampertico
Mar 1, 1996·Nature Medicine·Q P DouK Keyomarsi

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Citations

Jun 16, 2001·International Journal of Cancer. Journal International Du Cancer·Y DongM Tokuda
Jan 26, 2011·Medical Oncology·Asli NarBeyhan Demirhan
May 19, 2004·The International Journal of Biochemistry & Cell Biology·Tarik Möröy, Christoph Geisen
Feb 10, 2004·Gynecologic Oncology·Alaa A El-GhobashyC Simon Herrington
Aug 7, 1998·Contraception·D L Moyer, J C Felix
Nov 7, 1998·Molecular and Cellular Endocrinology·V PayraudeauV Albaladéjo
Mar 30, 2000·Cancer Letters·K MakiyamaM Hosaka
Nov 27, 2001·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Z E WintersC J Norbury
Dec 7, 2006·Journal of the National Cancer Institute·Peggy L PorterDaniel F Hayes
Mar 11, 2010·Applied Immunohistochemistry & Molecular Morphology : AIMM·Lukasz KuźbickiBarbara W Chwirot
Aug 1, 1997·APMIS : Acta Pathologica, Microbiologica, Et Immunologica Scandinavica·G Landberg, G Roos
Mar 17, 2001·Journal of Clinical Pathology·J GeorgievaD Schadendorf
May 29, 2002·Molecular Pathology : MP·C SimoneG Guanti
Mar 12, 1999·Annual Review of Medicine·J TsihliasJ Slingerland
Jan 14, 2010·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·James W PurcellSylvaine Cases
Jul 19, 2002·Pancreatology : Official Journal of the International Association of Pancreatology (IAP) ... [et Al.]·Yasuhiro ItoNariaki Matsuura
Dec 14, 2006·Breast Cancer Research : BCR·Mark J BeresfordAndreas Makris
Oct 19, 2004·American Journal of Clinical Pathology·Heike GrabschWolfram Mueller
Feb 12, 2002·Photochemistry and Photobiology·Arianna L KimJean Gautier
Jul 25, 2000·Biochemical and Biophysical Research Communications·R HaddadW A Held
Sep 18, 1997·Proceedings of the National Academy of Sciences of the United States of America·J M TrowbridgeM J Garabedian
Oct 29, 1997·Proceedings of the National Academy of Sciences of the United States of America·J LangenfeldE Dmitrovsky
Jan 17, 2012·Clinica Chimica Acta; International Journal of Clinical Chemistry·Li-nian HuangWei Jia
Aug 1, 2007·The Journal of Cell Biology·Jamie M KeckSteven I Reed
May 13, 2003·International Journal of Cancer. Journal International Du Cancer·Pierre RudolphReza Parwaresch
Oct 19, 2006·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·Elizabeth BallDavid Wynford-Thomas
Mar 16, 2007·Cancer Science·Takashi SuzukiHironobu Sasano
Aug 18, 2012·Cytometry. Part a : the Journal of the International Society for Analytical Cytology·Jiang MinJianping Gong
Mar 13, 2010·Cell Biology International·Ikue KikuchiNaoto Yamaguchi
Aug 9, 2002·Pathology, Research and Practice·Yasuhiro ItoAkira Miyauchi
Jul 11, 2002·The American Journal of Pathology·James A WohlschlegelAnindya Dutta

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