PMID: 6409386May 1, 1983Paper

Cyclo-oxygenase products of arachidonic acid metabolism in rat osteoblasts in culture

Calcified Tissue International
R D NolanT J Martin

Abstract

The metabolism of arachidonic acid to its cyclo-oxygenase products was studied in monolayer cultures of osteoblast-rich rat calvarial cells and of clonal cell lines from a rat osteogenic sarcoma, enriched in the osteoblast phenotype. Prostanoids were measured by radioimmunoassay after extraction of media and fractionation by high pressure liquid chromatography. In both normal and malignant osteoblasts the major cyclooxygenase product was 6-oxo-prostaglandin F1 alpha, the hydration product of prostacyclin, with lesser amounts of prostaglandin E2 and prostaglandin F2 alpha. No significant thromboxane B2 was detected. Prostaglandins are thought to have a local role in the regulation of bone resorption. These results point to the possible importance of prostacyclin either in bone resorption or in some other local function, e.g., regulation of bone blood flow.

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Citations

Feb 1, 1990·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·N N AliS J Jones
May 1, 1993·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·J R SandyR W Farndale
Nov 1, 1992·Calcified Tissue International·G Shankar, P H Stern
Jan 1, 1996·Histochemistry and Cell Biology·M MiyauchiN Ijuhin
Sep 1, 1985·Prostaglandins·N C PartridgeT J Martin
Feb 1, 1985·Prostaglandins·R W Walenga, W Bergstrom
Nov 1, 1992·Cellular Signalling·B OgisoC A McCulloch
Dec 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·J M WittenbergJ F Osborn
May 24, 2003·Biochemical and Biophysical Research Communications·A D BakkerE H Burger
Dec 28, 1999·Journal of Biomechanics·M JoldersmaJ Klein-Nulend
Feb 1, 1988·Baillière's Clinical Endocrinology and Metabolism·T J MartinG C Nicholson
Jan 26, 2007·American Journal of Physiology. Cell Physiology·Amanda M D MaloneChristopher R Jacobs
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·C H LohmannZ Schwartz
Oct 23, 2012·Bone·Jenneke Klein-NulendSheldon Weinbaum
Jan 1, 1996·Progress in Histochemistry and Cytochemistry·M Goldberg, A L Boskey
Jul 19, 2005·Journal of Biomechanics·Nikhil N BatraChristopher R Jacobs
Oct 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·A FujimoriT Fujita
Apr 1, 1989·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·V P MichelangeliT J Martin
Jan 1, 1989·Clinical Endocrinology·I R Reid
Mar 1, 1983·The Journal of Pathology·T J Chambers, N N Ali
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Mar 1, 1985·Journal of Clinical Pathology·T J Chambers
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Feb 15, 2001·American Journal of Physiology. Endocrinology and Metabolism·M JoldersmaE H Burger
Jan 1, 1992·The American Journal of Physiology·A M FreyaldenhovenM S Katz
Sep 8, 2001·Prostaglandins, Leukotrienes, and Essential Fatty Acids·I FortierA J de Brum-Fernandes

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