Cyclodextrin aided separation of peptides and proteins by capillary zone electrophoresis

Journal of Chromatography. a
A S Rathore, C Horváth

Abstract

Carboxymethylated-beta-cyclodextrin (CMBCD) in the electrophoretic medium (aqueous 50 mM sodium phosphate, pH 2.5) enhanced the separation using raw fused-silica capillaries in CZE of the four standard proteins: alpha-chymotrypsinogen A, cytochrome c, lysozyme and ribonuclease A. Furthermore, with 20 mM CMBCD in the electrophoretic medium, the cis-trans isomers of angiotensin could be separated at room temperature, whereas the separation of the conformers required subambient temperatures as low as -20 degrees C without CMBCD in the electrophoretic medium [50 mM sodium phosphate (pH 2.5), containing 10% (v/v) methanol]. Addition of heptakis(2,6-di-O-methyl)-beta-cyclodextrin (DMBCD) had no effect on the separation of the above proteins and peptides. The results suggest that in microcolumn separation techniques, certain cyclodextrin additives can be useful selectivity enhancers.

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·B Di BlasioG Vecchio
Jun 1, 1991·Analytical Chemistry·J K Towns, F E Regnier
Oct 24, 1986·Journal of Chromatography·D E Henderson, C Horváth
Jun 6, 1995·Biochemical and Biophysical Research Communications·N Karuppiah, A Sharma
Mar 21, 1994·FEBS Letters·P CamilleriD R Howlett
Feb 16, 1996·The Journal of Biological Chemistry·D Rozema, S H Gellman
Sep 13, 1996·Journal of Chromatography. a·F ThuneckeC Horváth
Oct 23, 1996·Biochemical and Biophysical Research Communications·F CouthonC Vial

❮ Previous
Next ❯

Citations

Sep 6, 2003·Journal of Chromatography. B, Analytical Technologies in the Biomedical and Life Sciences·Anna LupiAntonella Forlino
Feb 2, 2017·Beilstein Journal of Organic Chemistry·Loïc Leclercq
Dec 22, 1999·Electrophoresis·V Kasicka
Jul 10, 2021·International Journal of Biological Macromolecules·Xiao FanYong Wang

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bacterial Cell Wall Structure (ASM)

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.

Bacterial Cell Wall Structure

Bacterial cell walls are made of peptidoglycan (also called murein), which is made from polysaccharide chains cross-linked by unusual peptides containing D-amino acids. Here is the latest research on bacterial cell wall structures.