PMID: 8611612Jan 12, 1996Paper

Cyclodextrins: a new tool for the controlled lipid depletion of thylakoid membranes

Biochimica Et Biophysica Acta
A Rawyler, P A Siegenthaler

Abstract

Cyclodextrins (CDs) have been used in controlled lipid depletion of thylakoid membranes avoiding the use of either detergents or lipolytic enzymes. Spinach thylakoid membranes were first treated with different CDs under various conditions. After removal of the CDs by washing, the amounts of mono-- and digalactosyldiacylglycerol (MGDG and DGDG), sulfoquinovosyldiacylglycerol (SQDG) and phosphatidylglycerol (PG), protein, pigment and plastoquinone remaining in the membranes were determined. The main results, obtained with alpha-CD and heptakis-(2,6-di-O-methyl)-beta-CD (DM-beta-CD), were as follows. (1)Acyl lipids were removed from thylakoid membranes by both CDs (DM-beta-CD being more efficient than alpha-CD; the extent of removal depended on both CD and chlorophyll concentrations. (2) alpha-CD presented a higher selectivity towards lip classes than did DM-beta-CD, but in both cases the removal order was SQDG > PG > MGDG > DGDG. (3) alpha-CD showed a preference for those lipids containing saturated 16-carbon acyl chains whereas DM-beta-CD was essentially insensitive to the fatty acid composition of the lipids. (4) The protein, chlorophyll and carotenoid contents of thylakoids were not affected by CD treatments. (5) Plastoquinone...Continue Reading

References

Oct 10, 1975·Biochimica Et Biophysica Acta·Z Krupa, T Baszynski
Dec 8, 1989·European Journal of Biochemistry·Y OhtaniJ Pitha
Jan 1, 1987·European Journal of Immunology·H Ohmori, I Yamamoto
Mar 15, 1985·European Journal of Biochemistry·L VighG Horváth
Sep 1, 1980·European Journal of Biochemistry·A Rawyler, P A Siegenthaler

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Citations

Feb 1, 1997·Journal of Pharmaceutical Sciences·T Irie, K Uekama
Aug 2, 2015·Chemistry and Physics of Lipids·Manuela DenzPeter Müller
Sep 8, 2010·Biophysical Journal·Michael S JablinJarosław Majewski
May 25, 2010·Journal of Colloid and Interface Science·Michał FlasińskiPatrycja Dynarowicz-Łatka

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