Cyclodextrins as a chiral mobile phase additive in nano-liquid chromatography: comparison of reversed-phase silica monolithic and particulate capillary columns

Analytical and Bioanalytical Chemistry
Anna RoccoSalvatore Fanali

Abstract

Enantioseparations of racemic nonsteroidal anti-inflammatory drugs (naproxen, ibuprofen, ketoprofen, flurbiprofen, suprofen, indoprofen, cicloprofen, and carprofen) were performed by nano-liquid chromatography, employing achiral capillary columns and heptakis(2,3,6-tri-O-methyl)-β-cyclodextrin (TM-β-CD) or hydroxylpropyl-β-cyclodextrin (HP-β-CD) as a chiral mobile phase additive (CMPA). Working under the same experimental conditions (in terms of mobile phase and linear velocity), the performance of a RP-C18 monolithic column was compared with that of a RP-C18 packed column of the same dimensions (100 μm i.d. × 10 cm). Utilizing a mobile phase composed of 30% ACN (v/v) buffered with 50 mM sodium acetate at pH 3, and containing 30 mM TM-β-CD, the monolithic column provided faster analysis but lower resolution than the packed column. This behavior was ascribed to the high permeability of the monolithic column, as well as to its minor selectivity. HP-β-CD was chosen as an alternative to TM-β-CD. Employing the monolithic column, the effects of different parameters such as HP-β-CD concentration, mobile phase composition, and pH on the retention factor and the chiral resolution of the analytes were studied. For the most of the analyte...Continue Reading

References

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Jul 16, 2008·Journal of Separation Science·Giovanni D'Orazio, Salvatore Fanali

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Citations

Mar 16, 2016·Journal of Pharmaceutical Sciences·Alexey RyzhakovThorsteinn Loftsson
May 28, 2013·Journal of Pharmaceutical and Biomedical Analysis·Ghodratollah Absalan, Yousef Alipour
Jan 8, 2013·Journal of Separation Science·Anna RoccoSalvatore Fanali
Oct 22, 2020·Journal of Chromatography. a·Mohammad Mahdi Moein, Thuy A Tran

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