Cyclomaltodextrinase, neopullulanase, and maltogenic amylase are nearly indistinguishable from each other.

The Journal of Biological Chemistry
Hee-Seob LeeKwan-Hwa Park

Abstract

Over 20 enzymes denoted as cyclomaltodextrinase, maltogenic amylase, or neopullulanase that share 40-86% sequence identity with each other are found in public data bases. These enzymes are distinguished from typical alpha-amylases by containing a novel N-terminal domain and exhibiting preferential substrate specificities for cyclomaltodextrins (CDs) over starch. In this research field, a great deal of confusion exists regarding the features distinguishing the three groups of enzymes from one another. Although a different enzyme code has been assigned to each of the three different enzyme names, even a single differentiating enzymatic property has not been documented in the literature. On the other hand, an outstanding question related to this issue concerns the structural basis for the preference of these enzymes for CDs. To clarify the confusion and to address this question, we have determined the structures of two enzymes, one from alkalophilic Bacillus sp. I-5 and named cyclomaltodextrinase and the other from a Thermus species and named maltogenic amylase. The structure of the Bacillus enzyme reveals a dodecameric assembly composed of six copies of the dimer, which is the structural and functional unit of the Thermus enzyme ...Continue Reading

References

May 15, 1991·Analytical Biochemistry·J D Fox, J F Robyt
Mar 1, 1991·Acta Crystallographica. Section A, Foundations of Crystallography·T A JonesM Kjeldgaard
Oct 12, 1990·Biochimica Et Biophysica Acta·T OgumaK Mizusawa
Feb 23, 1996·Journal of Molecular Biology·G FiedlerA Böck
Aug 15, 1998·Nucleic Acids Research·Z ZhangS F Altschul
Oct 3, 1998·Acta Crystallographica. Section D, Biological Crystallography·A T BrüngerG L Warren
Sep 3, 1999·The Journal of Biological Chemistry·J S KimB H Oh
Apr 20, 2001·Journal of Protein Chemistry·B K TibbotG H Robertson
Sep 1, 1994·Acta Crystallographica. Section D, Biological Crystallography·UNKNOWN Collaborative Computational Project, Number 4
Jan 1, 1997·Methods in Enzymology·Zbyszek Otwinowski, Wladek Minor
Jan 1, 1997·Methods in Enzymology·Todd O Yeates

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Citations

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May 20, 2003·European Journal of Biochemistry·Hanna B FritzscheGeorg E Schulz
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