Cyclometalated Iridium(III) Complexes as AIE Phosphorescent Probes for Real-Time Monitoring of Mitophagy in Living Cells

Scientific Reports
Chengzhi JinHui Chao

Abstract

Mitophagy, which is a special autophagy that removes damaging mitochondria to maintain sufficient healthy mitochondria, provides an alternative path for addressing dysfunctional mitochondria and avoiding cellular death. In the present study, by coupling the triphenylamine group with 2-phenylimidazo[4,5-f][1,10]phenanthroline derivatives, we synthesized five Ir(III) complexes with an AIE property that are expected to fulfill requirements for real-time monitoring of mitophagy. Ir1-Ir5 were exploited to image mitochondria with a short incubation time by confocal microscopy and inductive coupled plasma-mass spectrometry (ICP-MS). Due to aggregation-induced emission (AIE), Ir1-Ir5 exhibited excellent photostability compared to MitoTracker Green (MTG). Moreover, Ir1-Ir5 manifested satisfactory photostability in the mitochondrial physiological pH range. In addition, the uptake mechanism of Ir1 was investigated using confocal microscopy and flow cytometry analysis. Finally, using both Ir1 and LysoTracker Green, we were able to achieve real-time monitoring of mitophagy.

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Citations

Mar 28, 2017·ACS Applied Materials & Interfaces·Mu-He ChenZong-Wan Mao
Jul 6, 2017·Small·Yao-Xin LinHao Wang
Oct 19, 2016·Dalton Transactions : an International Journal of Inorganic Chemistry·Kangqiang QiuHui Chao
Aug 17, 2017·Dalton Transactions : an International Journal of Inorganic Chemistry·Yue ZhengZong-Wan Mao
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Sep 7, 2016·Small·Yi-Feng WangXing-Jie Liang
Jan 29, 2021·Dalton Transactions : an International Journal of Inorganic Chemistry·Nilmadhab RoyAnbalagan Moorthy
Nov 7, 2020·Chemical Society Reviews·Siyang Ding, Yuning Hong
Nov 16, 2017·ACS Applied Materials & Interfaces·Ju MeiHe Tian
May 19, 2018·ACS Applied Materials & Interfaces·Anushree DuttaArun Chattopadhyay
Nov 28, 2021·ChemMedChem·Hanchen ShenBen Zhong Tang

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Methods Mentioned

BETA
confocal microscopy
phosphorescence
column chromatography
flow cytometry
nuclear magnetic resonance
ELISA
FACS

Software Mentioned

AxioVision

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