Cyclooxygenase- and lipoxygenase-dependent relaxation to arachidonic acid in rabbit small mesenteric arteries

American Journal of Physiology. Heart and Circulatory Physiology
David X ZhangWilliam B Campbell

Abstract

We recently reported that the lipoxygenase product 11,12,15-trihydroxyeicosatrienoic acid (THETA) mediates arachidonic acid (AA)-induced relaxation in the rabbit aorta. This study was designed to determine whether this lipoxygenase metabolite is involved in relaxation responses to AA in rabbit small mesenteric arteries. AA (10(-9)-10(-4) M) produced potent relaxations in isolated phenylephrine-preconstricted arteries, with a maximal relaxation of 99 +/- 0.5% and EC(50) of 50 nM. The cyclooxygenase (COX) inhibitors indomethacin (10 microM), NS-398 (10 microM, selective for COX-2), and SC-560 (100 nM, selective for COX-1) caused a marked rightward shift of concentration responses to AA. With the use of immunohistochemical analysis, both COX-1 and COX-2 were detected in endothelium and smooth muscle of small mesenteric arteries. Indomethacin-resistant relaxations were further reduced by the lipoxygenase inhibitors cinnamyl-3,4-dihydroxy-cyanocinnamate (CDC; 1 muM), nordihydroguaiaretic acid (NDGA; 1 microM), and ebselen (1 microM). HPLC analysis showed that [(14)C]AA was metabolized by mesenteric arteries to PGI(2), PGE(2), THETAs, hydroxyepoxyeicosatrienoic acids (HEETAs), and 15-hydroxyeicosatetraenoic acid (15-HETE). The produc...Continue Reading

References

Sep 1, 1991·The American Journal of Physiology·S L PfisterW B Campbell
Mar 1, 1988·British Journal of Pharmacology·M Feletou, P M Vanhoutte
Sep 1, 1973·Circulation Research·J T Herlihy, R A Murphy
Dec 1, 1995·Circulation·R A Cohen, P M Vanhoutte
Mar 1, 1996·The American Journal of Physiology·S L PfisterW B Campbell
Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·C J SmithP C Isakson
Oct 9, 1999·The American Journal of Pathology·U SchönbeckP Libby
Oct 16, 1999·Nature·B FisslthalerR Busse
Jun 19, 2001·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·F M FaraciN L Weintraub
Aug 18, 2001·The Journal of Immunology : Official Journal of the American Association of Immunologists·G E CaugheyM J James
Dec 20, 2002·The Journal of Pharmacology and Experimental Therapeutics·Allison W MillerNeal L Weintraub
Aug 9, 2003·American Journal of Physiology. Heart and Circulatory Physiology·William B CampbellSandra L Pfister
Nov 19, 2003·Archives of Biochemistry and Biophysics·Sandra L PfisterWilliam B Campbell
Dec 24, 2003·Hypertension·Kathryn M GauthierWilliam B Campbell

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Citations

Feb 26, 2008·Journal of the American Society for Mass Spectrometry·Lijie CuiKasem Nithipatikom
Jun 16, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Yuttana ChawengsubWilliam B Campbell
Mar 7, 2012·Journal of Receptor and Signal Transduction Research·Lei YuDaling Zhu
Mar 18, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Michel Félétou, Paul M Vanhoutte
Mar 28, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Robert M BryanSean P Marrelli
Oct 17, 2006·American Journal of Physiology. Heart and Circulatory Physiology·Nitin T AggarwalWilliam B Campbell
Aug 30, 2013·American Journal of Physiology. Renal Physiology·Bin LiuYingbi Zhou
Mar 6, 2007·American Journal of Physiology. Heart and Circulatory Physiology·David X ZhangWilliam B Campbell
Dec 20, 2012·Journal of Cardiovascular Pharmacology·William B Campbell, Kathryn M Gauthier
Nov 27, 2008·Journal of Cardiovascular Pharmacology·Aya TakakiHiroaki Shimokawa
Sep 29, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Nabil FoudiMagnus Bäck
Jul 13, 2007·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·Phoebe A StapletonJefferson C Frisbee
Jan 15, 2008·American Journal of Physiology. Heart and Circulatory Physiology·Yuttana ChawengsubWilliam B Campbell

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