Cyclooxygenase-2 Modulates Glycosaminoglycan Production in the Skin During Salt Overload

Frontiers in Physiology
Róbert AgócsAttila J Szabó

Abstract

Sodium (Na+) can accumulate in the skin tissue, sequestered by negatively charged glycosaminoglycans (GAGs). During dietary salt overload, the amount and charge density of dermal GAG molecules - e.g., hyaluronic acid (HA) and chondroitin sulfate (CS) - increases; however, the regulation of the process is unknown. Previously, it has been demonstrated that the level of cyclooxygenase-2 (COX-2) activity and the content of prostaglandin E2 (PGE2) are elevated in the skin due to high-salt consumption. A link between the COX-2/PGE2 system and GAG synthesis was also suggested. We hypothesized that in dermal fibroblasts (DFs) high-sodium concentration activates the COX-2/PGE2 pathway and also that PGE2 increases the production of HA. Our further aim was to demonstrate that the elevation of the GAG content is ceased by COX-2 inhibition in a salt overloaded animal model. For this, we investigated the messenger RNA (mRNA) expression of COX-2 and HA synthase 2 enzymes as well as the PGE2 and HA production of DFs by real-time reverse transcription PCR (qRT-PCR) and ELISA, respectively. The results showed that both high-sodium concentration and PGE2 treatment increases HA content of the media. Sodium excess activates the COX-2/PGE2 pathway i...Continue Reading

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Methods Mentioned

BETA
ELISA
reverse transcription PCR
PCR

Key Resources (RRID) Mentioned

RGD
SCR_012155
SCR_016295

Software Mentioned

QuanLynx
Lasergene PrimerSelect
GraphPad Prism
LightCycler 480
Waters MassLynx
GraphPad

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