Cyclooxygenase-2-regulated vascular endothelial growth factor release in gastric fibroblasts

American Journal of Physiology. Gastrointestinal and Liver Physiology
Shuhei MiuraChoitsu Sakamoto

Abstract

VEGF is a highly specific stimulator of endothelial cells and may play an important role in angiogenesis in the process of tissue regeneration. We previously showed that cyclooxygenase-2 (COX-2) expressed in mesenchymal cells of the ulcer bed is involved in the ulcer repair process. To clarify the role of COX-2 in angiogenesis during gastric ulcer healing, we investigated the relation between COX-2 expression and VEGF production in human gastric fibroblasts in vivo and in vitro. Gastric fibroblasts were cultured in RPMI 1640 with and without IL-1alpha or IL-1beta in the presence or absence of NS-398, a selective COX-2 inhibitor. Supernatant VEGF and PGE(2) concentrations were measured by enzyme-linked immunosorbent assay. COX-2 expression in fibroblasts was determined by Western blot analysis. VEGF and COX-2 expression in surgical resections of human gastric ulcer tissue was examined immunohistochemically. IL-1 dose dependently enhanced VEGF release in cultured gastric fibroblasts after a 24-h stimulation. IL-1 also stimulated PGE(2) production in gastric fibroblasts via COX-2 induction. NS-398 significantly suppressed VEGF and PGE(2) release from IL-1-stimulated gastric fibroblasts; concurrent addition of PGE(2) restored NS-39...Continue Reading

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Citations

Apr 6, 2007·Digestive Diseases and Sciences·Kikuko AmagaseKoji Takeuchi
Aug 11, 2010·Journal of Ethnopharmacology·Maria Silene da SilvaAlba Regina Monteiro Souza Brito
Jul 3, 2008·International Wound Journal·Andrej RingStefan Langer
Oct 17, 2014·Medicinal Research Reviews·Sharad Kumar Suthar, Manu Sharma
Jul 4, 2016·Medical Hypotheses·Ljubomir VitkovMatthias Hannig
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Oct 2, 2004·American Journal of Physiology. Gastrointestinal and Liver Physiology·Yoko ShinjiChoitsu Sakamoto
Nov 9, 2004·Laboratory Investigation; a Journal of Technical Methods and Pathology·Katya GudisChoitsu Sakamoto
Sep 27, 2005·Digestive Diseases and Sciences·Katya Gudis, Choitsu Sakamoto
Dec 17, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Kazuhiro NagataChoitsu Sakamoto
Dec 31, 2005·American Journal of Physiology. Gastrointestinal and Liver Physiology·Tsutomu NishidaNorio Hayashi
Sep 27, 2008·Journal of Oral Science·Demos G Kalyvas, Maria Tarenidou
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Aug 4, 2007·American Journal of Physiology. Gastrointestinal and Liver Physiology·Ryo HatazawaKoji Takeuchi
Feb 4, 2010·Bioorganic & Medicinal Chemistry·Ying WuHoon Cho

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