PMID: 2509384Jan 1, 1989Paper

Cyclooxygenase and lipoxygenase inhibitors act differently on oxidative product formation by immune mononuclear cells: a flow cytometric investigation

International Journal of Immunopharmacology
N RouahiJ C Mani

Abstract

The action of indomethacin, a cyclooxygenase inhibitor, and nordihydroguaiaretic acid (NDGA), a lipoxygenase inhibitor, on oxidative products formed by immune mononuclear cells was studied by flow cytometry. Jurkat T-cells and peripheral blood mononuclear cells were incubated with 2',7'-dichlorofluorescin diacetate; this substance is hydrolyzed in the cells leading to non-fluorescent 2',7'-dichlorofluorescin which is oxidized by oxygen reactive species into highly fluorescent 2,7'-dichlorofluorescein. Using this fluorescent probe, the formation of oxygen reactive species in phytohemagglutinin-stimulated mononuclear cells treated by NDGA or indomethacin was followed by flow cytometry. We observed that NDGA caused a marked decrease, both in the level of fluorescence intensity and in the number of fluorescent cells, whereas indomethacin caused a small increase in fluorescence intensity. On the other hand, NDGA inhibited and indomethacin increased the incorporation of tritiated thymidine by phytohemagglutinin-stimulated lymphocytes. These results suggest that oxygen reactive species are involved in the stimulation of immune mononuclear cells.

References

Jan 1, 1987·International Archives of Allergy and Applied Immunology·J WassermanI Vedin
Jan 1, 1987·Cytometry·S KinoshitaT Kushida
Jan 1, 1985·Immunology Letters·M GerberA Crastes de Paulet
Feb 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·A NovogrodskyK H Stenzel
Jan 1, 1984·Immunology Letters·A E KingstonJ E Kay
Mar 1, 1982·The Journal of Experimental Medicine·R S Rappaport, G R Dodge

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Citations

Jan 3, 2001·Free Radical Biology & Medicine·D M van ReykN H Hunt

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