Cyclooxygenase inhibitors enhance cell growth in an osteoblastic cell line, MC3T3-E1

Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research
A FujimoriT Fujita

Abstract

To elucidate the significance of endogenous prostaglandin E2 (PGE2) in osteoblastic cell function, we studied the effects of cyclooxygenase inhibitors on cell growth and alkaline phosphatase (ALP) activity in MC3T3-E1 cells. UMR-106 cells were also used as references in our experiments. MC3T3-E1 cells, cultured in alpha-minimal essential medium containing 10% fetal bovine serum, were shown to produce PGE2, which was markedly suppressed in the presence of indomethacin. Addition of indomethacin resulted in an increase in DNA content and [3H]thymidine incorporation. A similar growth stimulatory effect was observed when structurally different cyclooxygenase inhibitors, that is, acetyl salicylic acid (ASA), flurbiprofen, and piroxicam, were added. These cyclooxygenase inhibitors, however, differed in their effects on ALP activity. Indomethacin and ASA enhanced ALP activity, whereas flurbiprofen and piroxicam suppressed it. We then examined the effects of exogenous addition of PGE2. Although exogenous PGE2 at 6 x 10(-6) M slightly stimulated cell growth, it inhibited cell growth at 6 x 10(-8) M and 6 x 10(-7) M. ALP activity was reduced in a dose-dependent fashion by exogenous PGE2. These results suggest that PGE2 produced by MC3T3-E...Continue Reading

References

Dec 10, 1974·Prostaglandins·L G Raisz, A R Koolemans-Beynen
Oct 25, 1972·Nature: New Biology·N Blumenkrantz, J Sondergaard
Jan 28, 1970·Journal of the American Chemical Society·E J CoreyN M Weinshenker
Mar 1, 1980·Analytical Biochemistry·C Labarca, K Paigen
May 1, 1983·Calcified Tissue International·R D NolanT J Martin
Jan 1, 1984·Prostaglandins·Y S Chyun, L G Raisz
Aug 1, 1983·The Journal of Pediatrics·R E RingelM A Berman
Oct 30, 1981·Biochemical and Biophysical Research Communications·S B RodanL G Raisz

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Citations

Sep 11, 2003·Comparative Biochemistry and Physiology. Toxicology & Pharmacology : CBP·Rafael MateoDavid J Hoffman
Sep 1, 1996·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·B A WatkinsM F Seifert
May 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·M H HuoG E Friedlaender
Feb 7, 1998·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M KanematsuY Yamada
Mar 21, 2007·Journal of Cellular Physiology·Christina M LeeClare E Yellowley
Oct 31, 2003·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Shilpa ChoudharyCarol C Pilbeam
Feb 1, 2006·Basic & Clinical Pharmacology & Toxicology·Li-Ling TsengChung-Ren Jan
Nov 1, 1992·Cellular Signalling·B OgisoC A McCulloch

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