Cyclooxygenase inhibitors not inhibit resting lung cancer A549 cell proliferation

Prostaglandins, Leukotrienes, and Essential Fatty Acids
Weigang Duan, Luyong Zhang

Abstract

Cyclooxygenase (COX) inhibitors were regarded as anticarcinogenic agents for lung cancer at least partly via PGE2; but these were based on cytokin stimulation experiment on A549 cell. In order to clarify whether COX inhibitors directly inhibit A549 cell, three COX inhibitors, NS398 (selective COX-2 inhibitor), SC560 (selective COX-1 inhibitor), and acetyl salicylic acid (ASA, non-selective COX inhibitor), were studied. NS398, and ASA, can inhibit PGE2 generation via COX-2 inhibition. The viability of A549 cell was assayed by MTT. However, without cytokin stimulation, all the three inhibitors (NS398 0.2-20 microM; SC560 1.0-100 nM; ASA 0.01-1.0 mM) were not able to inhibit A549 cell proliferation, in the other way round, NS398 promoted cell growth. And arachidonic acid (AA) and lipopolysaccharide (LPS) did not disturb the property of its growth. These data suggested that without cytokin stimulation, COX and PGE2 may not be the kernel molecules involved in A549 cell proliferation, and COX inhibitors could not inhibit A549 cell growth directly.

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Citations

Feb 3, 2012·Journal of Asian Natural Products Research·Ze-Hua HuJi-Zhou Wu
Nov 6, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Yu YunZhi Qiang Shen
Mar 3, 2007·Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan·Jin Cheng YaoLu Yong Zhang
Jun 26, 2013·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Wei WangXiao-Hua Wang
Jul 8, 2009·Toxicology in Vitro : an International Journal Published in Association with BIBRA·Jan I HersethPer E Schwarze
Jun 21, 2008·Research in Veterinary Science·Hso-Chi ChaungWen-Bin Chung
Sep 22, 2016·Journal of Asian Natural Products Research·Qiang FuYu-Zhong Zheng
Nov 20, 2012·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Peng CaoGuobiao Liang
Jul 1, 2011·Expert Opinion on Therapeutic Patents·Baker Jawabrah Al-HouraniFrank Wuest
Sep 25, 2009·Journal of Natural Products·Tao FengXiao-Dong Luo
Jun 15, 2010·Journal of Natural Products·Qiang FuPengfei Tu

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