Cyclooxygenase isoenzyme localization and mRNA expression in rat lungs

American Journal of Respiratory Cell and Molecular Biology
L ErmertW Seeger

Abstract

Prostanoid generation may proceed via both isoforms of cyclooxygenase, Cox-1 and Cox-2. Cox-1 is thought to be ubiquitously expressed, whereas Cox-2 is mostly assumed to be dynamically regulated, responding to inflammatory stimuli. The cellular localization of Cox-1 and Cox-2 in the lung, an organ with high cyclooxygenase activity, is not known. In normal rat lungs the expression and localization of Cox-1 and Cox-2 were examined with immunogold-silver staining and the RT-PCR technique. Quantitative image analysis of the staining intensity was performed by measuring mean gray values of digitized epipolarization images. Expression of both Cox-1 and Cox-2 was readily detectable in rat lungs. Cox-1 immunoreactivity localized predominantly to bronchial epithelial cells, smooth muscle cells of large hilum veins, and (with lower expression) to alveolar macrophages and pulmonary artery endothelial cells. The most intense Cox-2 staining was noted in macrophage- and mast cell-like cells, detected in close vicinity to the bronchial epithelium and in the connective tissue surrounding the vessels. In addition, strong Cox-2 expression was found in smooth muscle cells of partially muscular vessels and large veins of the hilum. Bronchial epith...Continue Reading

References

Apr 1, 1975·Journal of Morphology·O Paes de AlmeidaA Paes de Carvalho
Oct 1, 1992·American Journal of Respiratory Cell and Molecular Biology·Y OhkawaraT Takishima
Jan 1, 1992·Annual Review of Physiology·M J Holtzman
Jul 9, 1992·The New England Journal of Medicine·B W ChristmanJ E Loyd
Jan 1, 1991·The American Review of Respiratory Disease·M J Holtzman
May 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·F C Thien, E H Walters
Mar 17, 1995·Biochemical and Biophysical Research Communications·S Arias-NegreteK Chadee
Apr 1, 1995·Prostaglandins, Leukotrienes, and Essential Fatty Acids·M Goppelt-Struebe
Mar 1, 1995·American Journal of Respiratory Cell and Molecular Biology·T EndoS Yamamoto
Dec 15, 1993·Biochemical and Biophysical Research Communications·B P KennedyW A Cromlish
Oct 18, 1994·Biochemical Pharmacology·T KleinV Ullrich
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·J A MitchellJ R Vane
Dec 1, 1993·Archives of Biochemistry and Biophysics·L FengD Hwang
Sep 13, 1993·FEBS Letters·G P O'Neill, A W Ford-Hutchinson
Apr 1, 1993·The Journal of Clinical Investigation·N SuttorpC Schudt
Mar 1, 1993·Cell and Tissue Research·L ErmertH R Duncker
Oct 1, 1995·Annals of Medicine·P J Barnes
Feb 1, 1997·American Journal of Respiratory and Critical Care Medicine·P DemolyJ Bousquet

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Citations

Jan 22, 2000·Arthritis and Rheumatism·L J CroffordL B van de Putte
Apr 24, 2008·Basic Research in Cardiology·Lukasz DrelicharzStefan Chlopicki
Sep 6, 2000·Clinical and Experimental Allergy : Journal of the British Society for Allergy and Clinical Immunology·J C Fernández-MorataC Picado
Feb 15, 2000·American Journal of Respiratory and Critical Care Medicine·R TahaA Giaid
Jan 31, 2002·American Journal of Respiratory and Critical Care Medicine·Tsuyoshi OgumaKazuhiro Yamaguchi
May 27, 2004·American Journal of Respiratory and Critical Care Medicine·Anna-Marie Bloch MünsterJohannes Sidelmann
Jun 5, 2002·American Journal of Respiratory and Critical Care Medicine·Kate G DavidsonIan R Doyle
Dec 4, 2003·American Journal of Respiratory and Critical Care Medicine·Andreas GüntherWerner Seeger
Feb 13, 2001·American Journal of Respiratory Cell and Molecular Biology·C MartinV Ullrich
Mar 23, 2011·American Journal of Respiratory Cell and Molecular Biology·Paul D Bozyk, Bethany B Moore
May 17, 2000·Pediatric Research·P LassusS Andersson
Jun 8, 2010·Cell Transplantation·Hiroshi YagiMartin L Yarmush
Jul 30, 2011·Antiviral Research·Suki M Y LeeJ S M Peiris
Feb 1, 2011·Pathophysiology : the Official Journal of the International Society for Pathophysiology·Zaher A Radi, Mark R Ackermann
Dec 31, 2008·Pulmonary Pharmacology & Therapeutics·Deborah L ClarkeAkira Yamasaki
Jul 1, 2008·Pharmacology & Therapeutics·Daphne MerkusDirk J Duncker
Jul 29, 2008·Journal of Cellular and Molecular Medicine·Nina ZidarDusan Stajer
Dec 12, 2001·Respiratory Research·A GüntherW Seeger
Jan 30, 2007·Respiratory Research·Ralph T SchermulyFrank Rose
Dec 12, 2012·European Journal of Pharmacology·Anna-Karin Larsson-CallerfeltChristian Martin
Sep 21, 2004·Respiratory Research·Anne-Karin HesseFritz Krombach
Feb 13, 2001·The American Journal of Pathology·L ErmertM Ermert
Jul 26, 2006·Free Radical Biology & Medicine·Peter F Bove, Albert van der Vliet
Nov 6, 2007·International Journal of Pharmaceutics·Kerstin LahnsteinTobias Gessler
Oct 19, 2001·Toxicological Sciences : an Official Journal of the Society of Toxicology·B C GottschlingA Nyska
Oct 31, 2000·Veterinary Pathology·K N KhanR K Harris
Nov 2, 2004·Journal of Applied Physiology·Candice D FikeSandra L Pfister
Mar 7, 2006·The Journal of Urology·Jana Jaal, Wolfgang Dörr

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