Cyclooxygenase metabolism of endogenous arachidonic acid by cultured human tracheal epithelial cells

The American Review of Respiratory Disease
L ChurchillD Proud

Abstract

The epithelial cell may contribute to the regulation of pulmonary function during inflammatory diseases of the airways by producing metabolites of arachidonic acid (AA). We have used human tracheal epithelial cells (HTE), grown in serum-free medium, to examine cyclooxygenase metabolism of endogenous AA by these cells. Gas chromatography-negative ion mass spectrometry demonstrated that, regardless of stimulus (buffer, bradykinin, or the calcium ionophore A23187), epithelial cells produce PGE2 and PGF2 alpha but no detectable levels of PGD2, thromboxane B2, 6-keto-PGF1 alpha, or 9 alpha, 11 beta-PGF2. Preincubation of cultures with medium containing 5% human serum led to striking increases in the production of PGE2 and PGF2 alpha, regardless of stimulus. Concomitant with these increases in prostanoids, serum exposure caused a 3.6-fold increase in total cellular arachidonate. Arachidonate levels increased in all phosphoglyceride classes, with the greatest increases in phosphatidylethanolamine, phosphatidylcholine, and phosphatidylinositol. In serum-pretreated cells, PGE2 production was 1.46 +/- 0.12, 4.74 +/- 0.6, and 6.35 +/- 0.93 ng/10(6) cells (mean +/- SEM; n = 7) upon exposure to buffer, 10(-6) M bradykinin, and 1 micrograms/...Continue Reading

References

Jan 1, 1988·The Journal of Infectious Diseases·R M NaclerioJ M Gwaltney
May 1, 1985·Journal of Applied Physiology·J H WiddicombeI K Tuet
Jul 11, 1985·The New England Journal of Medicine·R M NaclerioL M Lichtenstein
Mar 1, 1988·The American Review of Respiratory Disease·D ProudR M Naclerio
Jul 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·J A HunterM J Holtzman
Apr 1, 1985·The American Review of Respiratory Disease·L A LaitinenT Haahtela
Nov 1, 1985·British Journal of Pharmacology·P J BarnesJ B Palmer
Oct 1, 1984·Pathology·J S PedersenB H Toh
Jul 26, 1984·The New England Journal of Medicine·C C HardyS T Holgate
Aug 1, 1959·Canadian Journal of Biochemistry and Physiology·E G BLIGH, W J DYER

❮ Previous
Next ❯

Citations

Jul 1, 1991·Immunopharmacology·S G Farmer
Jun 16, 2000·European Journal of Pharmacology·J HjobergF P Nijkamp
Jun 9, 1999·The Journal of Allergy and Clinical Immunology·M O AksoyS G Kelsen
Aug 1, 1997·Trends in Pharmacological Sciences·S R Johnson, A J Knox
Sep 24, 1998·Trends in Pharmacological Sciences·G Folkerts, F P Nijkamp
Jan 1, 1997·Research in Immunology·D B Jacoby
Jun 30, 2000·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·H S ParkO J Kwon
May 5, 1999·Annals of Allergy, Asthma & Immunology : Official Publication of the American College of Allergy, Asthma, & Immunology·H S ParkD C Choi
Jul 1, 1997·British Journal of Pharmacology·N WatsonK F Rabe
Sep 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·M P Anderson, M J Welsh
Jan 16, 2009·Respirology : Official Journal of the Asian Pacific Society of Respirology·Siiri AltrajaAlan Altraja
Nov 17, 1998·American Journal of Respiratory and Critical Care Medicine·D Spina
Jan 5, 1999·American Journal of Respiratory and Critical Care Medicine·G M GauvreauP M O'Byrne
Dec 10, 1999·American Journal of Respiratory and Critical Care Medicine·R M KafouryM Friedman
Jul 1, 1993·The American Review of Respiratory Disease·I D PavordA E Tattersfield
Nov 1, 1994·American Journal of Respiratory and Critical Care Medicine·P H HowarthS T Holgate
Jul 29, 2008·American Journal of Respiratory and Critical Care Medicine·David ProudJeffrey W Clymer
Jul 1, 1997·American Journal of Respiratory Cell and Molecular Biology·L M SchwiebertR P Schleimer
Apr 1, 1999·American Journal of Respiratory Cell and Molecular Biology·J AtsutaR P Schleimer
Aug 26, 1999·American Journal of Respiratory Cell and Molecular Biology·M B Feinstein, R P Schleimer
Nov 26, 1999·American Journal of Respiratory Cell and Molecular Biology·M ArimaR P Schleimer
Mar 14, 2001·American Journal of Respiratory Cell and Molecular Biology·S P SandersD Proud
Feb 1, 1991·American Journal of Respiratory Cell and Molecular Biology·R P Schleimer

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.