Cyclophilin B enhances HIV-1 infection

Virology
Jason DeBoerMichael Belshan

Abstract

Cyclophilin B (CypB) is a member of the immunophilin family and intracellular chaperone. It predominantly localizes to the ER, but also contains a nuclear localization signal and is secreted from cells. CypB has been shown to interact with the Gag protein of human immunodeficiency type 1 (HIV-1). Several proteomic and genetic studies identified it as a potential factor involved in HIV replication. Herein, we show that over-expression of CypB enhances HIV infection by increasing nuclear import of viral DNA. This enhancement was unaffected by cyclosporine treatment and requires the N-terminus of the protein. The N-terminus contains an ER leader sequence, putative nuclear localization signal, and is required for secretion. Deletion of the N-terminus resulted in mislocalization from the ER and suppression of HIV infection. Passive transfer experiments showed that secreted CypB did not impact HIV infection. Combined, these experiments show that intracellular CypB modulates a pathway of HIV nuclear import.

References

May 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·S K SwansonF Rusnak
Apr 1, 1992·Immunology Today·S L Schreiber, G R Crabtree
Sep 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·A KarpasF Hill
Apr 26, 1994·Proceedings of the National Academy of Sciences of the United States of America·E R PriceF D McKeon
Nov 24, 1994·Nature·E K FrankeJ Luban
Nov 24, 1994·Nature·M ThaliH G Göttlinger
Mar 7, 1996·Biochimica Et Biophysica Acta·C MarillerG Spik
Oct 6, 1998·Annual Review of Biochemistry·A D Frankel, J A Young
Aug 10, 2000·Molecular Endocrinology·M A RycyzynC V Clevenger
May 9, 2002·Proceedings of the National Academy of Sciences of the United States of America·Michael A Rycyzyn, Charles V Clevenger
Mar 15, 2005·The Journal of Biological Chemistry·Yoko ObataToshifumi Matsuyama
Jul 2, 2005·Molecular Cell·Koichi WatashiKunitada Shimotohno
Sep 25, 2007·Biochemical and Biophysical Research Communications·Tatiana PushkarskyMichael Bukrinsky
Mar 7, 2009·PLoS Pathogens·Nan YanJudy Lieberman
Jun 6, 2009·Journal of Virological Methods·Michael BelshanAlan Engelman
Jul 25, 2009·PLoS Pathogens·Malgorzata Bienkowska-HabaMartin Sapp
Dec 10, 2009·PLoS Genetics·Jae Won ChoiRichard J Bram
Feb 2, 2011·Virology·Hiroto KambaraYoshiharu Matsuura
Jul 19, 2011·Journal of Molecular Biology·Mainul HoqueMichael B Mathews
Jul 19, 2012·PloS One·Cameron J SchweitzerMichael Belshan
Jan 4, 2013·Journal of Proteome Research·Cameron J SchweitzerMichael Belshan
Sep 23, 2014·Virology·Jason DeBoerMichael Belshan
Nov 8, 2014·Nucleic Acids Research·Danso Ako-AdjeiKim D Pruitt

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.

Aminoglycosides (ASM)

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.

Aminoglycosides

Aminoglycoside is a medicinal and bacteriologic category of traditional Gram-negative antibacterial medications that inhibit protein synthesis and contain as a portion of the molecule an amino-modified glycoside. Discover the latest research on aminoglycoside here.