Cyclophosphamide attenuates the degenerative changes induced by CSF from patients with amyotrophic lateral sclerosis in the neonatal rat spinal cord

Journal of the Neurological Sciences
N ShahaniT R Raju

Abstract

Our earlier studies have shown that cerebrospinal fluid (CSF) of patients with amyotrophic lateral sclerosis (ALS), when intrathecally injected into the neonatal rats, produces an aberrant phosphorylation of neurofilaments (NF) in the ventral horn neurons and reactive astrogliosis in the spinal cord. We wanted to investigate the effect of cyclophosphamide in the spinal cords of neonatal rats exposed to ALS-CSF. A single dose (5 microg in 5 microl saline) of cyclophosphamide was injected, 24 h after the administration of CSF samples from ALS and non-ALS neurological patients into the spinal subarachnoid space of 3-day-old rat pups. Rats were sacrificed after a period of 24 h, and stained with antibodies against the phosphorylated NF (SMI-31 antibody) and glial fibrillary acidic protein (GFAP). Cyclophosphamide treatment resulted in a 50% decrease in the number of SMI-31 stained neuronal soma in ventral horns of spinal cords of ALS-CSF exposed rats. This was accompanied by a decrease in the number of GFAP immunoreactive astrocytes. Furthermore, lactate dehydrogenase (LDH) activity was also decreased significantly, following cyclophosphamide treatment. These results suggest that cyclophosphamide could exert a neuroprotective effec...Continue Reading

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Citations

Dec 4, 2013·Clinical Proteomics·Anu Mary VargheseTrichur R Raju
Oct 23, 2013·Neurología : publicación oficial de la Sociedad Española de Neurología·U Gomez-PinedoA G García
Jan 13, 2005·Acta Neurologica Scandinavica·F J Jiménez-JiménezF Cabrera-Valdivia
Jul 28, 2011·International Journal of Cancer. Journal International Du Cancer·Mustafa Aziz HatibogluAmy B Heimberger
Jun 30, 2004·Journal of Neuroimmunology·David A BallokBoris Sakic
Jun 2, 2014·Molecular Neurobiology·K VijayalakshmiPhalguni Anand Alladi
Jun 3, 2011·Journal of the Neurological Sciences·Nobutoshi MorimotoKoji Abe
Aug 27, 2010·Neurología : publicación oficial de la Sociedad Española de Neurología·J Matías-GuiuA Guerrero
Oct 30, 2004·Amyotrophic Lateral Sclerosis and Other Motor Neuron Disorders : Official Publication of the World Federation of Neurology, Research Group on Motor Neuron Diseases·Neelam ShahaniT R Raju
Apr 6, 2012·Social Psychiatry and Psychiatric Epidemiology·Deepa RaoR Raguram
Apr 25, 2007·Amyotrophic Lateral Sclerosis : Official Publication of the World Federation of Neurology Research Group on Motor Neuron Diseases·Priti Y RamamohanT R Raju
Apr 6, 2021·Frontiers in Molecular Neuroscience·Koy Chong Ng Kee KwongArpan R Mehta

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