Cyclosporin A blocks induction of tumor necrosis factor-alpha in human B lymphocytes

Biochemical and Biophysical Research Communications
C S SmithW T Shearer

Abstract

The effects of cyclosporin A (CSP) on tumor necrosis factor-alpha (TNF-alpha) RNA levels and protein production in human B cells and a B cell line were studied. The ability of CSP to block induction of RNA was compared to its inhibition of protein production in response to anti-IgM, phorbol ester (PMA) and platelet-activating factor (PAF). PAF is a phospholipid which has recently been found to activate human B cells. CSP blocks PAF-induced TNF-alpha RNA from the Ramos cell line, as well as inhibiting the enhancement of TNF protein production from both freshly isolated and Ramos B cells. CSP also blocks anti-Ig induced TNF-alpha RNA and protein but does not inhibit PMA-induced TNF-alpha. We conclude that B cells, like T cells, have CSP-independent and CSP-dependent signaling pathways and that PAF signaling is dependent upon a CSP-sensitive factor.

Citations

Jul 28, 2005·Joint, Bone, Spine : Revue Du Rhumatisme·Denis MullemanPhilippe Goupille
Dec 1, 2007·Arthritis and Rheumatism·Philippe GoupilleJean-Pierre Valat
Aug 6, 2003·BioDrugs : Clinical Immunotherapeutics, Biopharmaceuticals and Gene Therapy·Hanns-Martin Lorenz
Aug 29, 2019·ACS Omega·Paula BertonRobin D Rogers

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