PMID: 7511575Feb 1, 1994Paper

Cyclosporin A enhances susceptibility of multi-drug resistant human cancer cells to anti-P-glycoprotein antibody-dependent cytotoxicity of monocytes, but not of lymphocytes

Japanese Journal of Cancer Research : Gann
S YanoT Ogura


Cyclosporin A (CsA) was previously found to bind to P-glycoprotein expressed on multidrug-resistant (MDR) cancer cells. In the present study, the effect of CsA on anti-P-glycoprotein monoclonal antibody (mAb)-dependent cell-mediated cytotoxicity (ADCC) against human MDR cells was examined. The ADCC reaction was assessed by 4-h 51Cr-release assay. Highly purified lymphocytes (> 99%) and monocytes (> 99%) obtained from blood mononuclear cells (MNC) of healthy donors were used as effector cells. CsA decreased the cytotoxic activity of MNC against MDR cells, but enhanced their ADCC activity in the presence of anti-P-glycoprotein mAb MRK16. Lymphocyte-mediated ADCC and natural killer activity against MDR cells were also suppressed by addition of CsA. CsA induced a significant dose-dependent increase in monocyte-mediated ADCC activity. Interestingly, pretreatment of MDR cancer cells, but not of monocytes, with CsA significantly enhanced ADCC activity mediated by monocytes, but not by lymphocytes. A CsA analog (PSC833) and FK-506, but not verapamil also increased the sensitivity of MDR cells to ADCC by monocytes. CsA did not affect the binding of monocytes to MDR cells in the presence of MRK16 mAb. These results indicate that CsA may ...Continue Reading


Jan 1, 1992·Cancer Chemotherapy and Pharmacology·M NaitoT Tsuruo
Jul 1, 1991·Journal of Veterinary Internal Medicine·J Stewart, N T Gorman
Oct 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·H Hamada, T Tsuruo
Jan 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·A T FojoI Pastan
Mar 1, 1985·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·D R BellV Ling
Jan 1, 1985·Pharmacology & Therapeutics·J R Riordan, V Ling
May 29, 1986·Biochimica Et Biophysica Acta·L MátyusS Damjanovich
Mar 1, 1988·Japanese Journal of Cancer Research : Gann·T Tsuruo
Sep 15, 1988·International Journal of Cancer. Journal International Du Cancer·S SoneT Ogura
Apr 1, 1986·The Journal of Clinical Investigation·L M SlaterS Gupta
Jun 1, 1986·Proceedings of the National Academy of Sciences of the United States of America·M M CornwellI Pastan
Mar 1, 1985·Somatic Cell and Molecular Genetics·S AkiyamaM M Gottesman
Feb 1, 1981·Transplantation·M IntronaA Mantovani

❮ Previous
Next ❯


Aug 27, 2003·Journal of Tongji Medical University = Tong Ji Yi Ke Da Xue Xue Bao·Z ChenR Ohno

❮ Previous
Next ❯

Related Concepts

Related Feeds

Cancer Biology: Molecular Imaging

Molecular imaging enables noninvasive imaging of key molecules that are crucial to tumor biology. Discover the latest research in molecular imaging in cancer biology in this feed.

Adrenocortical Carcinoma

Adrenocortical carcinoma (ACC) is a rare malignancy of the adrenal cortex, associated with a generally dismal prognosis owing to its aggressive behavior. Here are the latest discoveries pertaining to this disease.

Antibody-Dependent Cell Cytotoxicity

Antibody-dependent cellular toxicity refers to the lysis of a target cell by a non-sensitized effector cell of the immune system as a result of antibodies binding to the target cell membrane and engaging the Fc receptors on the immune effector cells. Find the latest research on antibody-dependent cellular toxicity here.