PMID: 7539138May 23, 1995Paper

Cyclosporin A potentiates the dexamethasone-induced mouse mammary tumor virus-chloramphenicol acetyltransferase activity in LMCAT cells: a possible role for different heat shock protein-binding immunophilins in glucocorticosteroid receptor-mediated gene expression

Proceedings of the National Academy of Sciences of the United States of America
J M RenoirE E Baulieu

Abstract

As previously observed for FK506, we report here that cyclosporin A (CsA) treatment of mouse fibroblast cells stably transfected with the mouse mammary tumor virus-chloramphenicol acetyltransferase (MMTV-CAT) reporter plasmid (LMCAT cells) results in potentiation of dexamethasone (Dex)-induced CAT gene expression. Potentiation by CsA is observed in cells treated with 10-100 nM Dex but not in cells treated with 1 microM Dex, a concentration of hormone which results in maximum CAT activity. At 10 nM Dex, 1-5 microM CsA provokes an approximately 50-fold increase in CAT gene transcription, compared with transcription induced by Dex alone. No induction of CAT gene expression is observed in cells treated with CsA or FK506 in the absence of Dex. The antisteroid RU 486 abolishes effects obtained in the presence of Dex. Using a series of CsA, as well as FK506, analogs, including some devoid of calcineurin phosphatase inhibition activity, we conclude that the potentiation effects of these drugs on Dex-induced CAT gene expression in LMCAT cells do not occur through a calcineurin-mediated pathway. Western-blotting experiments following immunoprecipitation of glucocorticosteroid receptor (GR) complexes resulted in coprecipitation of GR, hea...Continue Reading

References

Nov 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·D A PeattieM Benasutti
Jul 15, 1992·Proceedings of the National Academy of Sciences of the United States of America·I CallebautJ P Mornon
Nov 2, 1984·Science·R E HandschumacherD W Speicher
Jan 1, 1994·The Journal of Steroid Biochemistry and Molecular Biology·J M RenoirE E Baulieu
Nov 8, 1994·Proceedings of the National Academy of Sciences of the United States of America·C RadanyiE E Baulieu
Feb 1, 1995·The Journal of Steroid Biochemistry and Molecular Biology·Y M Ning, E R Sanchez
Aug 16, 1993·Biochemical and Biophysical Research Communications·B RyffelB Car

❮ Previous
Next ❯

Citations

Oct 16, 1996·Molecular & General Genetics : MGG·V A Vucich, C S Gasser
Apr 1, 1996·Steroids·M BeatoM Truss
Jul 1, 1997·The Journal of Steroid Biochemistry and Molecular Biology·H E MacLeanJ D Zajac
Mar 25, 2000·The Journal of Steroid Biochemistry and Molecular Biology·V PrimaP Lefebvre
Nov 8, 2002·Respirology : Official Journal of the Asian Pacific Society of Respirology·Yasuko YoshiokaYoshinosuke Fukuchi
Aug 5, 1998·Journal of Clinical Psychopharmacology·R I ShaderD J Greenblatt
Mar 11, 2008·Investigative Ophthalmology & Visual Science·Xinyu ZhangThomas Yorio
Aug 19, 2008·FEBS Letters·Tony C LuuWilliam K Chan
Aug 25, 2004·Current Medical Research and Opinion·Naoki KawaguchiTakamichi Hattori
Jun 12, 1995·Annals of the New York Academy of Sciences·E E Baulieu
Nov 3, 2011·The EMBO Journal·Taichiro IkiMasayuki Ishikawa
Sep 1, 1995·Molecular Medicine Today·M Thali
Jul 19, 1996·The Journal of Biological Chemistry·A Kralli, K R Yamamoto
Jan 10, 2018·Molecular Psychiatry·Ozge Gunduz-CinarAndrew Holmes
Dec 17, 2016·Journal of Applied Physiology·Syotaro ObiTeruo Inoue
Jan 13, 1997·Biochemical and Biophysical Research Communications·T IshizukaN Terada
Jul 31, 1998·Molecular and Cellular Endocrinology·R D MedhT J Schmidt
May 26, 2004·Cellular Signalling·William B PrattDonald B DeFranco

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.