CyDiv, a Conserved and Novel Filamentous Cyanobacterial Cell Division Protein Involved in Septum Localization

Frontiers in Microbiology
Dinka MandakovicMónica Vásquez

Abstract

Cell division in bacteria has been studied mostly in Escherichia coli and Bacillus subtilis, model organisms for Gram-negative and Gram-positive bacteria, respectively. However, cell division in filamentous cyanobacteria is poorly understood. Here, we identified a novel protein, named CyDiv (Cyanobacterial Division), encoded by the all2320 gene in Anabaena sp. PCC 7120. We show that CyDiv plays a key role during cell division. CyDiv has been previously described only as an exclusive and conserved hypothetical protein in filamentous cyanobacteria. Using polyclonal antibodies against CyDiv, we showed that it localizes at different positions depending on cell division timing: poles, septum, in both daughter cells, but also in only one of the daughter cells. The partial deletion of CyDiv gene generates partial defects in cell division, including severe membrane instability and anomalous septum localization during late division. The inability to complete knock out CyDiv strains suggests that it is an essential gene. In silico structural protein analyses and our experimental results suggest that CyDiv is an FtsB/DivIC-like protein, and could therefore, be part of an essential late divisome complex in Anabaena sp. PCC 7120.

References

Jan 1, 1988·Methods in Enzymology·J Elhai, C P Wolk
Jun 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·T P Hopp, K R Woods
Jun 26, 2002·Journal of Bacteriology·Olga A Koksharova, C Peter Wolk
Sep 10, 2002·Journal of Bacteriology·Olga A Koksharova, C Peter Wolk
Mar 11, 2003·Microbiology and Molecular Biology Reviews : MMBR·Jeffery ErringtonDirk-Jan Scheffers
May 8, 2004·Molecular Microbiology·Khalil MazouniFranck Chauvat
Sep 29, 2004·Toxicon : Official Journal of the International Society on Toxinology·Daniela CastroMónica Vásquez
Mar 19, 2005·Molecular Microbiology·Shin-Ya MiyagishimaKatherine W Osteryoung
Jul 12, 2005·Current Biology : CB·Nathan W Goehring, Jon Beckwith
Feb 3, 2006·Journal of Bacteriology·Samer SakrTania Arcondeguy
Mar 15, 2006·Cell·Edgar HuitemaPatrick H Viollier
Aug 29, 2006·Trends in Microbiology·Enrique FloresIris Maldener
Nov 14, 2006·International Review of Cytology·Elizabeth HarryLyndal Thompson
Mar 3, 2007·Annual Review of Biochemistry·Joe Lutkenhaus
May 17, 2007·Molecular Microbiology·Tatyana RomantsovJanet M Wood
Feb 12, 2008·Current Protocols in Molecular Biology·K Wilson
Nov 8, 2008·Molecular Biology and Evolution·Fabia U Battistuzzi, S Blair Hedges
May 30, 2009·The EMBO Journal·Rok LenarcicLeendert W Hamoen
Aug 25, 2009·BMC Structural Biology·Martial MarboutyFranck Chauvat
Sep 10, 2009·Molecular Microbiology·Martial MarboutyFranck Chauvat
Dec 17, 2009·Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences·Thomas Cavalier-Smith
May 11, 2010·Cold Spring Harbor Perspectives in Biology·David Z Rudner, Richard Losick
Apr 5, 2011·Trends in Microbiology·Shailendra P Singh, Beronda L Montgomery
Sep 1, 2012·Molecular Microbiology·Anthony G VecchiarelliBarbara E Funnell
Dec 12, 2012·Annals of the New York Academy of Sciences·Alexander J F Egan, Waldemar Vollmer
Jun 12, 2013·The Journal of Cell Biology·Géraldine Laloux, Christine Jacobs-Wagner
Dec 7, 2013·BMC Systems Biology·Antti HäkkinenAndre S Ribeiro
Mar 8, 2014·BMC Plant Biology·Shin-ya MiyagishimaTakayuki Fujiwara
Jul 9, 2014·The Journal of Cell Biology·Anke Treuner-Lange, Lotte Søgaard-Andersen
Nov 2, 2015·Molecular Neurodegeneration·Rafaa ZeineddineJustin J Yerbury

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Methods Mentioned

BETA
confocal microscopy
PCR
light microscopy

Software Mentioned

SOMPA
CyDiv
ITASSER
PRED
blast
CyDiv BLAST
PSI
GenScript
Delta

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