PMID: 1092Dec 18, 1975

Cylic nucleotide phosphodiesterase in silkworm. Characterization of cyclic GMP phosphodiesterase

Biochimica Et Biophysica Acta
I Morishima


The existence of cyclic GMP phosphodiesterase (EC 3.1.4.-) was demonstrated in silkworm larva by gel filtration of the homogenate. The cyclic GMP phosphodiesterase was separated from cyclic AMP phosphodiesterases by column chromatography on hydroxyapatite and Sephadex G-200. The enzyme has a molecular weight of approx. 260 000, and optimum pH of 8.3 and a Km value of 2 muM. The enzyme is activated by 5 mM of Mg2+ and 2 mM of Mn2+. The cyclic GMP phosphodiesterase activity was greatly inhibited by low concentrations of cyclic IMP but to a lesser extent by cyclic AMP even at a high concentration. The activity was also inhibited by caffeine and theophylline.

Related Concepts

Cyclic AMP, (R)-Isomer
Molecular Sieve Chromatography
Cyclic GMP
Hydrogen-Ion Concentration
Cyclic IMP
Phosphoric diester hydrolase

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