CYP1/2 Activation and Glutathione-dependent Cytotoxicity of Four Pesticides in Hep G2 and Fa32 Cells

Toxicology in Vitro : an International Journal Published in Association with BIBRA
P J Dierickx

Abstract

The cytotoxicity of the carbamate insecticide carbaryl, the organophosphate insecticide quinalphos, the benzimidazole fungicide benomyl, and its debutylcarbamoylated derivative carbendazim was investigated in rat (Fa32) and human (Hep G2) hepatoma-derived established cell lines. Benomyl was the most toxic of the four pesticides, followed by quinalphos, carbaryl and the least toxic carbendazim, suggesting that the butylcarbamoyl group plays an important role in the toxicity of benomyl. EROD (7-ethoxyresorufin-O-deethylase) and PROD (7-pentoxyresorufin-O-depentylase) activity were moderately activated in Fa32 cells. In Hep G2 cells no PROD was measured, but EROD was activated 2.5 to 28 times the control values. Piperonyl butoxide and 1-aminobenzotriazole did not influence the cytotoxicity of the pesticides. However, when the endogenous glutathione content was reduced by pretreatment of the cells with l-buthionine-S,R-sulfoximine, the cytotoxicity of the pesticides strongly increased in both cell lines. In conclusion, the same cytotoxicity was observed for the four pesticides in both the animal and the human cell line. CYP1/2-dependent enzymes were activated to different degrees. No evidence was obtained for a cytochrome P450-depe...Continue Reading

References

Aug 1, 1985·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·E Borenfreund, C Shopsis
Dec 28, 1995·Toxicology·E HodgsonP E Levi
Oct 1, 1997·Toxicology in Vitro : an International Journal Published in Association with BIBRA·G de SousaR Rahmani

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Citations

Jul 19, 2011·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·L RibonnetY Larondelle
Jan 30, 2008·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Thérèse SergentYves-Jacques Schneider
Jan 1, 2006·Toxicology Mechanisms and Methods·Deniz YildizHaydar Oztas
Mar 30, 2010·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·E MaranM J Ruiz
Mar 11, 2008·Ecotoxicology and Environmental Safety·Elisa MaranMaría José Ruiz

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