CYP24A1 depletion facilitates the antitumor effect of vitamin D3 on thyroid cancer cells

Experimental and Therapeutic Medicine
Ning Hu, Hao Zhang

Abstract

It has been demonstrated that 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1) is a key enzyme that neutralizes vitamin D activity, which may have an anti-tumor effect. Therefore, the aim of the current study was to explore the effect of the active metabolite of vitamin D, 1,25-dihydroxyvitamin D (1,25-D3) on thyroid cancer cells following the downregulation of CYP24A1. A Cell Counting Kit-8 assay identified that CYP24A1 knockdown enhanced the anti-proliferative effects of 1,25-D3 on thyroid cancer cells. Furthermore, the results of the scratch wound and Transwell assays indicated that CYP24A1 knockdown enhanced the inhibitory effect of 1,25-D3 on cell migration. The results from reverse transcription-quantitative polymerase chain reaction and western blot analysis indicated that treatment with 1,25-D3 and CYP24A1 knockdown synergistically enhanced the expression of the epithelial-related gene E-cadherin and decreased the expression of the mesenchymal-related genes N-cadherin and vimentin. Following CYP24A1 knockdown and treatment with 1,25-D3, the expression of matrix metalloproteinase 2 and metalloproteinase inhibitor 1 were significantly decreased and increased, respectively, compared with the group that underwent treatment wit...Continue Reading

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Citations

Jul 19, 2020·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Ahmed El-Sharkawy, Ahmed Malki

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Methods Mentioned

BETA
transfection
electrophoresis
protein assay

Software Mentioned

ImageJ
GraphPad Prism

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