PMID: 9429230Jan 16, 1998Paper

CYP2D6 genotype and phenotyping by determination of dextromethorphan and metabolites in serum of healthy controls and of patients under psychotropic medication

Pharmacogenetics
D KöhlerChristoph Hiemke

Abstract

Fourteen drug free healthy volunteers and 22 psychiatric patients under psychotropic medication were phenotyped for their individual CYP2D6 activity using dextromethorphan as a probe drug. A solution containing 20 mg dextromethorphan was administered and blood was taken 60 min later for determination of dextromethorphan and metabolites in serum. For comparison, urine was collected over 8 h after ingestion of 20 mg dextromethorphan in a separate test. The CYP2D6 phenotype was determined from the ratio of dextromethorphan to dextrorphan. For genotyping, mutant alleles of the CYP2D6 gene were identified using allele-specific polymerase chain reactions. Genotyping revealed five poor metabolizers of CYP2D6. The others were extensive metabolizers. The ratio of dextromethorphan to dextrorphan ranged from 0.01-2.53 in serum and from 0.0007-4.252 in urine. Probit analysis of serum ratios revealed a bimodal distribution with an antimode at 0.126. According to this antimode, control subjects exhibited identical phenotypes and genotypes, whereas patients under paroxetine, moclobemide or metoprolol who had been genotyped as extensive metabolizers were poor metabolizer phenotypes. Administration of tricyclic antidepressants did not change th...Continue Reading

Citations

Aug 21, 2003·Journal of Biomedical Science·Geng-Chang YehMing-Thau Sheu
Jan 20, 2005·European Journal of Clinical Pharmacology·Minoo AfsharShahram Ala
Feb 3, 2007·European Journal of Clinical Pharmacology·D FrankU Fuhr
Oct 13, 2009·Clinical Pharmacokinetics·Shu-Feng Zhou
Jan 19, 2016·Pharmacogenomics·Rashmi R ShahRobert L Smith
Nov 17, 2004·Journal of Clinical Pharmacology·Mahmut OzdemirAmin Rostami-Hodjegan
May 3, 2012·Journal of Clinical Pharmacy and Therapeutics·J JuricaO Zendulka
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