CYP2E1 mRNA expression, genetic polymorphisms in peripheral blood lymphocytes and liver abnormalities in Chinese VCM-exposed workers

International Journal of Occupational Medicine and Environmental Health
Ai-Hong WangZhao-Lin Xia

Abstract

To study the relationship between expression of cytochrome P4502E1 (CYP2E1) in human lymphocytes, variant CYP2E1 genotype, exposure to vinyl chloride monomer (VCM), and liver abnormalities in VCM-exposed workers. A case-control study was performed on 90 male occupationally exposed workers and 42 matched male nonexposed controls. Data were collected based on health surveillance, workplace investigation and questionnaire Survey. Total RNA and DNA were isolated from peripheral blood lymphocytes, and CYP2E1 mRNA expression was determined using RT-PCR, and the presence of CYP2E1 polymorphisms was identified based on PCR-RFLP. The mRNA expression of CYP2E1 in exposed workers (0.89+/-0.46) was significantly higher than in nonexposed controls (0.61+/-0.35) (P < 0.01). Logistic regression analysis demonstrated a statistically significant association between CYP2E1 mRNA expression levels and liver abnormalities in the VCM-exposed workers (OR = 3.66, P < 0.05). The genotype frequency for CYP2E1 variants among VCM-exposed workers was not significantly different between workers with liver abnormalities and those without. Liver abnormalities in subjects exposed to VCM are positively associated with expression of peripheral blood lymphocyte m...Continue Reading

References

Jun 11, 1975·Environmental Health Perspectives·R E HefnerP J Gehring
Oct 1, 1990·The Journal of Clinical Endocrinology and Metabolism·B J SongP Saenger
Jan 1, 1982·Toxicology and Applied Pharmacology·J T DuC H Tamburro
Dec 1, 1995·Alcoholism, Clinical and Experimental Research·J L RaucyS P Carpenter
Oct 14, 1996·Biochemical and Biophysical Research Communications·Y SohB J Song
Mar 27, 2002·Biological & Pharmaceutical Bulletin·Fusako NagaiHiro-Omi Tamura
Jun 1, 2002·Occupational and Environmental Medicine·R-H WongT-J Cheng
Oct 23, 2002·Critical Reviews in Toxicology·Janne HukkanenHannu Raunio
Mar 27, 2007·Journal of Human Genetics·Jennifer SchindlerPaul W Brandt-Rauf

❮ Previous
Next ❯

Citations

Aug 19, 2010·International Archives of Occupational and Environmental Health·Tiancheng WangSen Zhen
Feb 8, 2011·Regulatory Toxicology and Pharmacology : RTP·Hana R Pohl, Franco Scinicariello
Oct 23, 2013·Drug Metabolism and Drug Interactions·Martha Sosa-Macías, Adrián Llerena

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.