PMID: 9187259Jun 1, 1997Paper

CYP2J subfamily cytochrome P450s in the gastrointestinal tract: expression, localization, and potential functional significance

Molecular Pharmacology
D C ZeldinS Wu

Abstract

Our laboratory recently described a new human cytochrome P450 arachidonic acid epoxygenase (CYP2J2) and the corresponding rat homologue (CYP2J3), both of which were expressed in extrahepatic tissues. Northern analysis of RNA prepared from the human and rat intestine demonstrated that CYP2J2 and CYP2J3 mRNAs were expressed primarily in the small intestine and colon. In contrast, immunoblotting studies using a polyclonal antibody raised against recombinant CYP2J2 showed that CYP2J proteins were expressed throughout the gastrointestinal tract. Immunohistochemical staining of formalin-fixed, paraffin-embedded intestinal sections using anti-CYP2J2 IgG and avidin-biotin-peroxidase detection revealed that CYP2J proteins were present at high levels in nerve cells of autonomic ganglia, epithelial cells, intestinal smooth muscle cells, and vascular endothelium. The distribution of this immunoreactivity was confirmed by in situ hybridization using a CYP2J2-specific antisense RNA probe. Microsomal fractions prepared from human jejunum catalyzed the NADPH-dependent metabolism of arachidonic acid to epoxyeicosatrienoic acids as the principal reaction products. Direct evidence for the in vivo epoxidation of arachidonic acid by intestinal cyto...Continue Reading

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Citations

Oct 19, 2002·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Shigeki MatsumotoYasushi Yamazoe
Apr 16, 2004·The Journal of Pharmacology and Experimental Therapeutics·Tracy C DeLozierJoyce A Goldstein
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Mar 24, 2009·American Journal of Physiology. Heart and Circulatory Physiology·Jeffrey J IliffNabil J Alkayed
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Apr 1, 2018·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Hsia-Lien LinPaul F Hollenberg
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Mar 12, 2008·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Doreen MitschkeUrsula Moenning
Jan 24, 2007·American Journal of Respiratory Cell and Molecular Biology·Caroline MorinEric Rousseau
Sep 12, 2013·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Eric A EvangelistaRheem A Totah
Jun 11, 2016·Drug Metabolism Reviews·Michael Murray
Jun 7, 2015·Drug Metabolism and Disposition : the Biological Fate of Chemicals·Eunyoung LeeKwang-Hyeon Liu
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Jul 18, 2018·International Journal of Molecular Sciences·Pierre LafiteDaniel Mansuy

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