CYP4BN6 and CYP6BQ11 mediate insecticide susceptibility and their expression is regulated by Latrophilin in Tribolium castaneum

Pest Management Science
Wenfeng XiongBin Li

Abstract

Many insect cytochrome P450 proteins (CYPs) are involved in the metabolic detoxification of exogenous compounds such as plant toxins and insecticides. Tribolium castaneum, the red flour beetle, is a major agricultural pest that damages stored grains and cereal products. With the completion of the sequencing of its genome, two T. castaneum species-specific CYP genes, CYP4BN6, and CYP6BQ11, were identified. However, it is unknown whether the functions of most CYPs are shared by TcCYP4BN6 and TcCYP6BQ11, and the upstream regulatory mechanism of these two CYPs remains elusive. QRT-PCR analysis indicated that TcCYP4BN6 and TcCYP6BQ11 were both most highly expressed at the late pupal stage and were mainly observed in the head and gut, respectively, of adults. Moreover, the transcripts of these two CYPs were significantly induced by dichlorvos and carbofuran, and RNA interference (RNAi) targeting of each of them enhanced the susceptibility of beetles to these two insecticides. Intriguingly, knockdown of the latrophilin (lph) gene, which has been reported to be related to the insecticide susceptibility, reduced the expression of TcCYP4BN6 and TcCYP6BQ11 after insecticide treatment, suggesting that these two CYP genes are regulated by l...Continue Reading

References

Oct 28, 1998·Proceedings of the National Academy of Sciences of the United States of America·T D SutherlandR Feyereisen
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·S F Steinberg, L L Brunton
Feb 13, 2002·Current Biology : CB·Gregor BucherMartin Klingler
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Feb 26, 2004·Proceedings of the National Academy of Sciences of the United States of America·Xianchun LiMary A Schuler
Jul 24, 2004·Journal of Neuroendocrinology·G SimonetJ Vanden Broeck
Sep 17, 2004·Nature·Gunter Meister, Thomas Tuschl
Nov 29, 2005·Journal of Insect Physiology·Julian A T Dow, Shireen A Davies
Aug 24, 2006·Annual Review of Entomology·Xianchun LiMay R Berenbaum
May 19, 2007·Science·Vishvanath NeneDavid W Severson
Mar 26, 2008·Nature·UNKNOWN Tribolium Genome Sequencing ConsortiumGregor Bucher
Sep 1, 2009·Insect Biochemistry and Molecular Biology·Khurshida BegumYoonseong Park
Sep 4, 2009·Comparative Biochemistry and Physiology. Part D, Genomics & Proteomics·Roger S HolmesJohn L Vandeberg
Apr 23, 2010·Proceedings of the National Academy of Sciences of the United States of America·Fang ZhuSubba R Palli
Dec 24, 2010·Journal of Medical Entomology·Ting Li, Nannan Liu
Jan 12, 2011·Insect Biochemistry and Molecular Biology·Guodong NiuMay R Berenbaum
Apr 15, 2011·British Journal of Pharmacology·P A InselA Wilderman
Sep 8, 2011·Genes & Development·Jyoti R MisraCarl S Thummel
Dec 31, 2011·Journal of Invertebrate Pathology·R R James, J Xu
Apr 17, 2012·Trends in Pharmacological Sciences·Brian K Shoichet, Brian K Kobilka
Oct 9, 2013·Insect Biochemistry and Molecular Biology·Jyoti R MisraCarl S Thummel
Oct 25, 2013·International Journal of Biological Sciences·Youhui GongNannan Liu
Jan 31, 2014·Archives of Insect Biochemistry and Physiology·Guo Xia LiuBalaji Rajashekar

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Citations

May 14, 2020·Current Pharmaceutical Design·Janin RösnerHans Merzendorfer
Jan 17, 2021·International Journal of Biological Macromolecules·Yuan-Chen ZhangKun-Peng Zhang

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