Cyr61 and Fisp12 are both ECM-associated signaling molecules: activities, metabolism, and localization during development

Experimental Cell Research
M L KireevaL F Lau

Abstract

cyr61 and fisp12 are homologous immediate-early genes that are transcriptionally activated upon growth factor stimulation in fibroblasts. Their gene products belong to an emerging family of secreted proteins with a high degree of sequence homology, including conservation of all 38 cysteine residues in their secreted portions. We have recently shown that Cyr61 is an extracellular matrix (ECM) signaling molecule that promotes cell proliferation, migration, and adhesion. We describe herein the first purification of the Fisp12 protein and we compare the activities of purified Cyr61 and Fisp12, their metabolism, targeting, and their localization during development. Although Fisp12 is the mouse homolog of the human connective tissue growth factor (CTGF), it has no detectable mitogenic activity by itself. Rather, Fisp12 enhances fibroblast growth factor-induced DNA synthesis. The activities of Fisp12 and Cyr61 are nearly indistinguishable in three cell types tested: fibroblasts, endothelial, and epithelial cells. Both proteins are found in the ECM, although Cyr61 associates with the ECM more strongly and binds heparin with higher affinity. Fisp12, but not Cyr61, is also found in the culture medium, suggesting that Fisp12 might be able...Continue Reading

References

Jun 1, 1991·Journal of Molecular and Cellular Cardiology·A F LauD S Crow
Jan 1, 1991·Annual Review of Neuroscience·L F Reichardt, K J Tomaselli
Jan 1, 1991·Annual Review of Biochemistry·H R Herschman
May 1, 1991·DNA and Cell Biology·A BrunnerA F Purchio
Jun 1, 1991·The Journal of Cell Biology·C Nathan, M Sporn
Jul 1, 1990·Molecular and Cellular Biology·T P O'BrienL F Lau
Feb 1, 1989·Proceedings of the National Academy of Sciences of the United States of America·D L SimmonsR L Erikson
Dec 1, 1987·The Journal of Cell Biology·U HeineM B Sporn
Apr 14, 1995·Science·E A Clark, J S Brugge

❮ Previous
Next ❯

Citations

May 16, 2008·Journal of Cell Communication and Signaling·Jennifer A JonesJohn J Castellot
May 16, 2008·Journal of Cell Communication and Signaling·Mark R GrayJohn J Castellot
Apr 13, 2011·Journal of Cell Communication and Signaling·Laure RittiéGary J Fisher
Mar 20, 2012·Journal of Cell Communication and Signaling·Véronique Ouellet, Peter M Siegel
Jul 23, 2002·Nature Cell Biology·José G AbreuE M De Robertis
Feb 16, 2008·The Journal of Biological Chemistry·Daphne PalaAndrew Leask
Sep 7, 2002·Human Gene Therapy·Virginie FataccioliEmmanuel Teiger
Jan 13, 2006·Clinical and Experimental Pharmacology & Physiology·Janette K Burgess
Jan 7, 2005·Journal of Thrombosis and Haemostasis : JTH·S K MandalU R Pendurthi
Nov 26, 2002·Molecular and Cellular Biology·Fan-E MoLester F Lau
Nov 4, 2005·Cancer Research·Usha R PendurthiL Vijaya Mohan Rao
Mar 29, 2006·Hormone Research·Sabine E HannemaIeuan A Hughes
Nov 2, 2006·Hormone Research·Sabine E Hannema, Ieuan A Hughes
Jan 2, 2007·American Journal of Respiratory Cell and Molecular Biology·Shu WuEduardo Bancalari
Apr 21, 2007·American Journal of Respiratory Cell and Molecular Biology·Gustavo Matute-BelloThomas R Martin
May 23, 2003·American Journal of Respiratory Cell and Molecular Biology·Mitsuyoshi UtsugiMasatomo Mori
May 30, 1998·Proceedings of the National Academy of Sciences of the United States of America·A M BabicL F Lau
May 1, 2004·Proceedings of the National Academy of Sciences of the United States of America·Eric M TamChristopher M Overall
Apr 26, 2000·Proceedings of the National Academy of Sciences of the United States of America·C H RedfernB R Conklin
Jul 16, 2013·Expert Opinion on Therapeutic Targets·Andrew Leask
Nov 13, 2012·FEBS Letters·Eriko AoyamaMasaharu Takigawa
Oct 16, 2015·International Journal of Molecular Medicine·Yu DiXiaolong Chen
Jun 7, 2000·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·M HadjiargyrouC T Rubin

❮ Previous
Next ❯

Related Concepts

Related Feeds

Adhesion Molecules in Health and Disease

Cell adhesion molecules are a subset of cell adhesion proteins located on the cell surface involved in binding with other cells or with the extracellular matrix in the process called cell adhesion. In essence, cell adhesion molecules help cells stick to each other and to their surroundings. Cell adhesion is a crucial component in maintaining tissue structure and function. Discover the latest research on adhesion molecule and their role in health and disease here.

Cardiovascular Homeostasis

Cardiovascular regulation and homeostasis is important in maintaining health and dysfunction may lead to cardiovascular diseases. Nitric oxide and vascular endothelium are one of many molecules involved in the homeostatic mechanism. Here is the latest research on cardiovascular homeostasis.