PMID: 9421523Feb 28, 1998Paper

Cys2/His2 zinc-finger protein family of petunia: evolution and general mechanism of target-sequence recognition

Nucleic Acids Research
K KuboH Takatsuji

Abstract

The EPF family is a group of Cys2/His2zinc-finger proteins in petunia. In these proteins, characteristically long spacer regions have been found to separate the zinc fingers. Our previous DNA-binding studies demonstrated that two-fingered proteins (ZPT2-1 and ZPT2-2), which have spacers of different lengths, bind to two separate AGT core motifs in a spacing specific manner. To investigate the possibility that these proteins might distinguish between the target sequences on the basis of spacing between the core motifs, we screened petunia cDNA library for other proteins belonging to this family. Initial screening by PCR and subsequent cloning of full-length cDNAs allowed us to identify the genes for 10 new proteins that had two, three or four zinc fingers. Among the two-fingered proteins the spacing between zinc fingers varied from 19 to 65 amino acids. The variation in the length of spacers was even more extensive in three- and four-fingered proteins. The presence of such proteins is consistent with our hypothesis that the spacing between the core motifs might be important for target sequence recognition. Furthermore, comparison of diverse protein structures suggests that three- and two-fingered proteins might have resulted due...Continue Reading

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Citations

Apr 15, 2005·Plant Molecular Biology·Rachel Holmes-DavisPhilip D Gregory
Aug 24, 2012·PloS One·Santosh Kumar GuptaTilak R Sharma
Jun 6, 2014·PloS One·Muhammed Jamsheer K, Ashverya Laxmi
Feb 9, 2012·Journal of Amino Acids·Setsuko FukushimaHiroshi Takatsuji
May 9, 2014·Plant Physiology and Biochemistry : PPB·Susan D LawrenceMichael B Blackburn
Feb 15, 2000·Genes to Cells : Devoted to Molecular & Cellular Mechanisms·S TsuchimotoE Ohtsubo
Sep 23, 2014·Plant Cell Reports·Lili WangWenchao Huang
Mar 17, 2004·Nucleic Acids Research·Akiko KozakiJoseph Colasanti
May 13, 2010·Journal of Experimental Botany·Shu-Jing SunHong-Sheng Zhang
Mar 4, 2011·DNA Research : an International Journal for Rapid Publication of Reports on Genes and Genomes·Delphine GourcilleauNathalie Leblanc-Fournier
Nov 9, 2000·The Plant Journal : for Cell and Molecular Biology·A IidaK Oeda
Feb 14, 2012·Plant Science : an International Journal of Experimental Plant Biology·Agnieszka Kiełbowicz-Matuk
May 1, 2005·Molecular Plant Pathology·Sang-Keun OhDoil Choi
Mar 5, 2003·Chembiochem : a European Journal of Chemical Biology·Carla IserniaRoberto Fattorusso
Jul 6, 2012·The Plant Journal : for Cell and Molecular Biology·Lijun AnYinbo Gan
Feb 29, 2008·BMB Reports·Eun Soo Seong, Myeong-Hyeon Wang
Oct 9, 2016·Plant Science : an International Journal of Experimental Plant Biology·Hongping ChangGang-Ping Xue
Jul 29, 1998·The Plant Journal : for Cell and Molecular Biology·A KobayashiH Takatsuji
Mar 15, 2013·International Journal of Molecular Sciences·Søren LindemoseKaren Skriver

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