Cyst Fluid Biosignature to Predict Intraductal Papillary Mucinous Neoplasms of the Pancreas with High Malignant Potential

Journal of the American College of Surgeons
Ajay V MakerStefan Green

Abstract

Current standard-of-care technologies, such as imaging and cyst fluid analysis, are unable to consistently distinguish intraductal papillary mucinous neoplasms (IPMNs) of the pancreas at high risk of pancreatic cancer from low-risk IPMNs. The objective was to create a single-platform assay to identify IPMNs that are at high risk for malignant progression. Building on the Verona International Consensus Conference branch duct IPMN biomarker review, additional protein, cytokine, mucin, DNA, and microRNA cyst fluid targets were identified for creation of a quantitative polymerase chain reaction-based assay. This included messenger RNA markers: ERBB2, GNAS, interleukin 1β, KRAS, MUCs1, 2, 4, 5AC, 7, prostaglandin E2R, PTGER2, prostaglandin E synthase 2, prostaglandin E synthase 1, TP63; microRNA targets: miRs 101, 106b, 10a, 142, 155, 17, 18a, 21, 217, 24, 30a, 342, 532, 92a, and 99b; and GNAS and KRAS mutational analysis. A multi-institutional international collaborative contributed IPMN cyst fluid samples to validate this platform. Cyst fluid gene expression levels were normalized, z-transformed, and used in classification and regression analysis by a support vector machine training algorithm. From cyst fluids of 59 IPMN patients,...Continue Reading

Citations

Aug 4, 2020·Journal of Cellular and Molecular Medicine·Shunda WangYupei Zhao
Feb 6, 2020·Scandinavian Journal of Surgery : SJS : Official Organ for the Finnish Surgical Society and the Scandinavian Surgical Society·A CaravatiG Marchegiani
Feb 14, 2020·International Journal of Molecular Sciences·Scarlett HaoAlexander A Parikh
Dec 5, 2019·Minerva chirurgica·Tommaso PolliniRoberto Salvia
Nov 5, 2019·Biochimica Et Biophysica Acta. Reviews on Cancer·Joseph CarmichealSurinder K Batra
May 6, 2021·Revista Española De Enfermedades Digestivas : Organo Oficial De La Sociedad Española De Patología Digestiva·Ana García García de ParedesEnrique Vázquez-Sequeiros
Jun 9, 2021·Nature Reviews. Gastroenterology & Hepatology·Aatur D Singhi, Laura D Wood
Jul 31, 2021·World Journal of Gastroenterology : WJG·Robert S O'Neill, Alina Stoita

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Ductal

Ductal carcinoma is a malignant neoplasm involving the ductal systems of any of a number of organs, such as the mammary glands, pancreas, prostate or lacrimal gland. Discover the latest research on ductal carcinoma here.