PMID: 5903098Jan 1, 1966

Cyst formation and poly-beta-hydroxybutyric acid accumulation in Azotobacter

Journal of Bacteriology
L H Stevenson, M D Socolofsky

Abstract

Stevenson, L. H. (Louisiana State University, Baton Rouge), and M. D. Socolofsky. Cyst formation and poly-beta-hydroxybutyric acid accumulation in Azotobacter. J. Bacteriol. 91:304-310. 1966.-The relationship between cyst formation and the accumulation of poly-beta-hydroxybutyric acid (PHB) in Azotobacter vinelandii (A. agilis) was investigated. After various periods of growth, the cells were harvested, and the amount of PHB and the extent of encystment were determined. The polymer content of the cells increased sharply and reached a maximum on the 2nd day of growth followed by a gradual decline as the culture aged. At maximal accumulation, the PHB content was 35% of the dry weight, and the PHB-nitrogen ratio was 11:1. Those substrates promoting the highest polymer content (glucose, butanol) also promoted 95 to 100% encystment. Manipulation of the carbon and nitrogen supply in the medium indicated that both the maximal PHB content and the extent of cyst formation could be controlled. A direct correlation was noted between the amount of polymer accumulated and the percentage of cysts formed, indicating a possible role of PHB as a carbon or energy source, or both, for the encystment process.

Citations

Nov 2, 1976·Archives of Microbiology·T FukuiT Saito
Aug 4, 2011·Microbial Ecology·Asli I Özen, David W Ussery
Jan 1, 1973·Antonie van Leeuwenhoek·L H Stevenson, M D Socolofsky
Jan 1, 1997·Critical Reviews in Biotechnology·F Clementi
May 8, 2016·Journal of Industrial Microbiology & Biotechnology·Lucero JiménezCarlos Peña
Mar 13, 2001·Environmental Microbiology·H K HøidalS Valla
Feb 9, 2017·Applied Microbiology and Biotechnology·Philippa HauschildAlexander Steinbüchel
Jan 11, 2011·Molecular Microbiology·Jeremiah N MardenCarl E Bauer
Mar 14, 2018·Microbiology·Jesse D Noar, Jose M Bruno-Bárcena
Jun 20, 2020·Metabolic Engineering Communications·Diego Tec CamposKarsten Zengler

Related Concepts

Azotobacter
Glucose, (beta-D)-Isomer
Hydrogen-Ion Concentration
Hydroxybutyric Acids
Lipid Metabolism

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