Cystathionine γ-lyase(CSE)/hydrogen sulfide system is regulated by miR-216a and influences cholesterol efflux in macrophages via the PI3K/AKT/ABCA1 pathway

Biochemical and Biophysical Research Communications
Duo GongChao-Ke Tang

Abstract

This study was designed to evaluate whether CSE/H2S system, which is regulated by miR-216a, regulated ABCA1-mediated cholesterol efflux and cholesterol contents in THP-1 macrophages-derived foam cells. Our qPCR and western blotting results showed that CSE/H2S significantly up-regulated the expression of ATP-binding cassette transporter A1 (ABCA1) mRNA and protein via PI3K/AKT pathway in foam cells derived from human THP-1 macrophages. The miR-216a directly targeted 3' untranslated region of CSE. It significantly reduced CSE and ABCA1 expression, and also decreased the phosphorylation of PI3K and AKT. Additionally, cholesterol efflux decreased, and cholesterol levels increased in THP-1 macrophage-derived foam cells in response to treatment with miR-216a. Our study demonstrates that CSE/H2S system is regulated by miR-216a, and regulates ABCA1-mediated cholesterol efflux and cholesterol levels through the PI3K/AKT pathway.

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Citations

Feb 22, 2017·Cellular Physiology and Biochemistry : International Journal of Experimental Cellular Physiology, Biochemistry, and Pharmacology·Tao DingHaiyan Hu
Apr 12, 2017·Antioxidants & Redox Signaling·Shuai YuanChristopher G Kevil
Dec 26, 2016·Atherosclerosis·Xiao-Dan XiaChao-Ke Tang
Dec 1, 2017·Antioxidants & Redox Signaling·María A Pajares, Dolores Pérez-Sala
Mar 8, 2018·Journal of Cellular and Molecular Medicine·Shujun YangWeili Zhang
Jun 17, 2020·Journal of Cellular Physiology·Yuqi ChenXiang Zhou
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Apr 4, 2021·International Journal of Molecular Sciences·Amin JavadifarAmirhossein Sahebkar

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