Cystathionine gamma-lyase is a component of cystine-mediated oxidative defense in Lactobacillus reuteri BR11.

Journal of Bacteriology
Raquel LoPhilip M Giffard

Abstract

Lactobacillus reuteri BR11 possesses a novel mechanism of oxidative defense involving an abundant cystine ABC transporter encoded by the cyuABC gene cluster. Large amounts of thiols, including H(2)S, are secreted upon cystine uptake by the CyuC transporter. A cystathionine gamma-lyase (cgl) gene is cotranscribed with the cyu genes in several L. reuteri strains and was hypothesized to participate in cystine-mediated oxidative defense by producing reducing equivalents. This hypothesis was tested with L. reuteri BR11 by constructing a cgl mutant (PNG901) and comparing it to a similarly constructed cyuC mutant (PNG902). Although Cgl was required for H(2)S production from cystine, it was not crucial for oxidative defense in de Mann-Rogosa-Sharpe medium, in contrast to CyuC, whose inactivation resulted in lag-phase arrest in aerated cultures. The importance of Cgl in oxidative defense was seen only in the presence of hemin, which poses severe oxidative stress. The growth defects in aerated cultures of both mutants were alleviated by supplementation with cysteine (and cystine in the cgl mutant) but not methionine, with the cyuC mutant showing a much higher concentration requirement. We conclude that L. reuteri BR11 requires a high con...Continue Reading

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Citations

Aug 28, 2012·Microbial Cell Factories·Sarang Dilip PophalySudhir Kumar Tomar
Jan 26, 2017·The Journal of Microbiology·Minwook KimSung-Jae Lee
Aug 16, 2017·Proceedings of the National Academy of Sciences of the United States of America·Philip H ChoiLiang Tong
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Sep 14, 2018·Frontiers in Microbiology·Chiara MontanariGiulia Tabanelli
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Oct 26, 2018·Journal of Agricultural and Food Chemistry·Bowen YanMichael Gänzle
Sep 24, 2014·Journal of Proteome Research·Xuan He, Carolyn M Slupsky

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