PMID: 6409085Apr 1, 1983Paper

Cystatin, a protein inhibitor of cysteine proteinases. Improved purification from egg white, characterization, and detection in chicken serum

The Biochemical Journal
A AnastasiA J Barrett

Abstract

The protein from chicken egg white that inhibits cysteine proteinases, and has been named 'cystatin', was purified by ovomucin precipitation, affinity chromatography on carboxymethylpapain-Sepharose and chromatofocusing. The final purification step separated two major forms of the protein (pI 6.5 and 5.6), with a total recovery of about 20% from egg white. By use of affinity chromatography and immunodiffusion it was shown that the inhibitor is also present at low concentrations in the serum of male and female chickens. Tryptic peptide maps of the separated forms 1 and 2 of egg-white cystatin were closely similar, and each form had the N-terminal sequence Ser-Glx-Asx. The two forms showed complete immunological identity, and neither contained carbohydrate. Ki values for the inhibition of cysteine proteinases were as follows: papain (less than 1 X 10(-11)M), cathepsin B (8 X 10(-10)M), cathepsin H (about 2 X 10(-8)M) and cathepsin L (about 3 X 10(-12)M). Some other cysteine proteinases, and several non-cysteine proteinases, were found not to be significantly inhibited by cystatin. The inhibition of the exopeptidase dipeptidyl peptidase I by cystatin was confirmed and the Ki found to be 2 X 10(-10)M. Inhibitor complexes with activ...Continue Reading

Citations

May 26, 2012·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·H K A PremachandraJehee Lee
Nov 26, 2008·Protein Expression and Purification·Nives SkrljMarko Dolinar
Jan 8, 2008·Developmental and Comparative Immunology·Christophe LefebvreClaude Cocquerelle
Jan 9, 2007·Protein Expression and Purification·Catharine C CalkinsKamiar Moin
Feb 6, 2009·Biotechnology Progress·Carolina W RibeiroFlavio Henrique-Silva
Aug 14, 2008·The FEBS Journal·Ulf EkströmFrancesc X Avilés
Jun 2, 2012·Journal of the Science of Food and Agriculture·Milica M PopovicMarija Gavrovic-Jankulovic
Jun 7, 2013·The Journal of Pharmacy and Pharmacology·Bogusława KonopskaMaria Warwas
Apr 24, 2013·Animal Science Journal = Nihon Chikusan Gakkaihō·Krzysztof GolabMaria Warwas
Jul 14, 2004·Biochemical and Biophysical Research Communications·Maria Luiza Vilela OlivaFlavio Henrique-Silva
Feb 6, 2007·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Bogusława KonopskaMaria Warwas
Jul 4, 2006·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Saroat RawdkuenTyre C Lanier
Nov 1, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Z SadafB Bilqees
Dec 7, 2005·Protein Expression and Purification·Andréia GianottiFlávio Henrique-Silva
Sep 6, 2005·Brain Research Bulletin·Keiji NishiyamaHisatsugu Koshimizu
Apr 28, 2004·Biochemical and Biophysical Research Communications·Luís Roberto de Camargo Gonçalves, Ana Marisa Chudzinski-Tavassi
Jan 25, 2011·Journal of Insect Physiology·Andrea Soares-CostaFlávio Henrique-Silva
Nov 26, 2010·The International Journal of Biochemistry & Cell Biology·Isel PascualMaría de los Angeles Chávez
Jul 27, 2000·Protein Expression and Purification·B RogeljM A Jongsma
Sep 15, 2006·Microbiological Research·Katarzyna Gawlik, Jan Gutowicz
Dec 1, 1995·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·J GburekM Warwas
Jun 1, 2012·Ticks and Tick-borne Diseases·Alexandra SchwarzMichalis Kotsyfakis
Apr 12, 2005·Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology·Eiichi SaitohShoji Odani
May 24, 2000·Proceedings of the National Academy of Sciences of the United States of America·R A StaniforthJ P Waltho
Feb 21, 1989·Biochemistry·I BjörkK Ylinenjärvi
Feb 1, 1993·Journal of Protein Chemistry·G LalmanachF Gauthier
Jul 1, 1996·Archives of Dermatological Research·H TsushimaH Mine
Jan 1, 1989·Experimental Cell Research·Q Sun

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.