Cystatin C is highly expressed in the human male reproductive system

Journal of Andrology
Thomas JibornAnders Bjartell

Abstract

Cystatin C displays the strongest inhibitory activity of all cystatins toward lysosomal cysteine proteases in general and has a widespread distribution in human tissues and body fluids, including seminal plasma. The aim of this study was to investigate the distribution of cystatin C in the male reproductive system. Immunohistochemistry revealed a widespread distribution of cystatin C in normal tissues from the testis, epididymis, vas deferens, seminal vesicle, and prostate gland. Immunoreactive cystatin C was localized in basal and secretory epithelial cells, but also in neuroendocrine cells in the prostate, identified by immunostaining for chromogranin A. On adjacent tissue sections, we demonstrated local production of cystatin C utilizing nonradioactive in situ hybridization with a 201-base-long digoxigenin-labeled antisense RNA probe specific for the cystatin C transcript. Staining patterns obtained by immunohistochemistry and in situ hybridization correlated well. Enzyme-linked immunosorbent assay for quantitative analysis of cystatin C demonstrated that cystatin C was present at high concentrations in tissue homogenates from all locations investigated, compared to liver, muscle, spleen, and other general tissues. Western b...Continue Reading

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Citations

Jul 9, 2010·International Journal of Andrology·L CarlssonA Larsson
Nov 9, 2011·Journal of Pediatric Gastroenterology and Nutrition·Behairy E BehairyMostafa M Sira
Jan 31, 2018·Reproductive Biology and Endocrinology : RB&E·Robert Kuo-Kuang LeeSheng-Hsiang Li
Oct 12, 2010·The Journal of International Medical Research·N KotajimaM Murakami
Oct 26, 2012·ISRN Obstetrics and Gynecology·Carmen Rodriguez-CerdeiraAlfonso Alba
May 3, 2005·Molecular Human Reproduction·Tamao NakanishiKaoru Suzumori
Sep 10, 2016·PloS One·Ganapathy Narmadha, Suresh Yenugu
May 7, 2014·The Journal of Clinical Endocrinology and Metabolism·Domenico MilardiAlfredo Pontecorvi
Dec 14, 2018·Molecular and Cellular Endocrinology·Christoph SchmidMarkus Niessen

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