PMID: 9192165Jun 1, 1997Paper

Cysteine and ascorbate loss in the diabetic rat lens prior to hydration changes

Current Eye Research
K P MittonJ R Trevithick

Abstract

Glutathione (GSH) loss precedes vacuole formation in the diabetic rat lens, but the cause of this loss is not known. Cysteine availability is a rate limiting factor to glutathione biosynthesis in rat and human lenses but its concentration is not known; therefore free cysteine was measured prior to lens hydration in the diabetic rat lens. GSH can regenerate ascorbate from dehydroascorbate within the lens and potentially modulate the ascorbate pool; therefore ascorbate loss is also a possibility that has not been examined previously. Diabetes was induced in Wistar rats to provide a slowly progressing model of cortical cataract. Age-matched control rats were injected with buffer vehicle only. Lens condition was monitored by binocular slit-lamp microscope after pupil dilation. Lens cysteine and glutathione were measured in the same lens, while ascorbate and total ascorbate (ascorbate + dehydroascorbate) of the contralateral lens were quantified by high performance liquid chromatography electrochemical detection. The 1- and 2-week periods of diabetes were chosen as they both preceded lens hydration changes and Na+/K+ changes, to avoid leakage due to ruptured cell membranes. Lens weights were not significantly different compared to c...Continue Reading

References

Apr 1, 1988·Experimental Eye Research·M F LouB M York
May 9, 1980·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Okamura
Jun 1, 1962·Experimental Eye Research·R VAN HAYNINGEN

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Citations

Mar 29, 2007·Molecular Nutrition & Food Research·Nandita ShangariPeter J O'Brien
Jan 5, 1999·FASEB Journal : Official Publication of the Federation of American Societies for Experimental Biology·A Y Lee, S S Chung
Dec 17, 2002·Clinical & Experimental Optometry : Journal of the Australian Optometrical Association·Arthur Stevens
Nov 30, 2000·Experimental Eye Research·P Chévez-BarriosM W Lieberman
Apr 3, 2001·Clinica Chimica Acta; International Journal of Clinical Chemistry·P M Abuja, R Albertini
Mar 23, 2000·Biochimica Et Biophysica Acta·G L Simpson, B J Ortwerth
Sep 6, 2021·International Journal of Clinical Practice·Şahbender Koç
Dec 24, 2010·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yosuke NakazawaMakoto Takehana

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