Cysteine cathepsins B and X promote epithelial-mesenchymal transition of tumor cells

European Journal of Cell Biology
Ana MitrovićJanko Kos

Abstract

Cathepsins B and X are lysosomal cysteine carboxypeptidases suggested as having a redundant role in cancer. They are involved in a number of processes leading to tumor progression but their role in the epithelial-mesenchymal transition (EMT) remains unknown. We have investigated the contribution of both cathepsins B and X in EMT using tumor cell lines differing in their expression of epithelial and mesenchymal markers and cell morphology. Higher levels of both cathepsins are shown to promote EMT and are associated with the mesenchymal-like cell phenotype. Moreover, simultaneous knockdown of the two peptidases triggers a reverse, mesenchymal to epithelial transition. Of the two cathepsins, cathepsin B appears to be the stronger promotor of EMT. Furthermore, we evaluated the involvement of cathepsin B and X in the transforming growth factor-β1 (TGF-β1) signaling pathway, one of the key signaling mechanisms triggering EMT in cancer. In MCF-7 cells the expression of cathepsin B was shown to depend on their activation with TGF-β1 while, for cathepsin X, a TGF-β1 independent mechanism of induction during EMT is indicated. EMT is thus shown to be another mechanism linking cathepsins B and X with tumor progression. With silencing of th...Continue Reading

Citations

May 9, 2018·Journal of Cancer Research and Clinical Oncology·Uljana A BoyarskikhMaxim L Filipenko
Oct 28, 2018·Radiology and Oncology·Barbara BreznikTamara T Lah
Sep 24, 2019·Journal of Microencapsulation·Jing SunJing Wu
Sep 5, 2019·Cancer Metastasis Reviews·Julia MitschkeThomas Reinheckel
Jul 30, 2019·PLoS Computational Biology·Majed Mohamed MagzoubOlivier Gevaert
Apr 2, 2020·Expert Opinion on Therapeutic Targets·Matej VizovišekBoris Turk
Jun 13, 2020·Cell Biochemistry and Function·Song ParkSeong-Kyoon Choi
Jul 27, 2018·Journal of Molecular Histology·Barbara BreznikCornelis J F Van Noorden
Apr 16, 2019·Developmental Cell·Arash LatifkarMarc A Antonyak
May 16, 2021·Journal of Hematology & Oncology·Ziqi ZhangXiawei Wei
Jun 20, 2020·Biochimica Et Biophysica Acta. Molecular Cell Research·Bernarda MajcTamara T Lah
Dec 30, 2021·Biomedit︠s︡inskai︠a︡ khimii︠a︡·T A GureevaN I Solovyova

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