Cysteine Dioxygenase Type 1 Inhibits Osteogenesis by Regulating Wnt Signaling in Primary Mouse Bone Marrow Stromal Cells

Scientific Reports
Xuefeng ZhaoEung-Kwon Pae

Abstract

Mesenchymal stem cells (MSCs) are multipotent cells, which can give rise to variety of cell types, including adipocytes and osteoblasts. Previously, we have shown that cysteine dioxygenase type 1 (Cdo1) promoted adipogenesis of primary mouse bone marrow stromal cells (BMSCs) and 3T3-L1 pre-adipocytes via interaction with Pparγ. However, the role of Cdo1 in osteogenesis remains unclear. Here, we demonstrated that expression of Cdo1 was elevated during osteoblastic differentiation of BMSCs in vitro. Interestingly, knockdown of Cdo1 by siRNA led to an increased expression of osteogenic related genes, elevated alkaline phosphatase (ALP) activity, and enhanced mineralization. Overexpression of Cdo1 in BMSCs inversely suppressed the osteogenesis. Furthermore, we found that overexpression of Cdo1 impaired Wnt signaling and restricted the Wnt3a induced expression of osteogenic transcriptional factors, such as Runx2 and Dlx5. Collectively, our findings indicate Cdo1 suppresses osteogenic differentiation of BMSCs, through a potential mechanism which involves in Wnt signaling reduction concomitantly.

References

Sep 1, 1991·Journal of Orthopaedic Research : Official Publication of the Orthopaedic Research Society·A I Caplan
Mar 13, 2003·Proceedings of the National Academy of Sciences of the United States of America·Huilin QiCatherine M Verfaillie
Oct 21, 2004·Blood·Sudeepta Aggarwal, Mark F Pittenger
Apr 26, 2005·The Journal of Biological Chemistry·Jennifer A Kennell, Ormond A MacDougald
Sep 28, 2005·Birth Defects Research. Part C, Embryo Today : Reviews·Tania M SchroederJennifer J Westendorf
May 4, 2007·Molecular and Cellular Endocrinology·Tatjana SchillingNorbert Schütze
Jul 10, 2007·Nature Medicine·Mone Zaidi
Apr 10, 2008·Cell Stem Cell·Paolo BiancoPaul J Simmons
Nov 18, 2008·Amino Acids·M H StipanukL L Hirschberger
Jan 10, 2009·Nature Protocols·Masoud Soleimani, Samad Nadri
Jan 13, 2009·Gene·Ling LingSimon M Cool
Oct 17, 2009·Journal of Molecular Cell Biology·Ryohichi Sugimura, Linheng Li
Mar 1, 2009·International Journal of Oral Science·Li PengXue-dong Zhou
Sep 30, 2010·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Edgardo Rodríguez-CarballoFrancesc Ventura
Dec 2, 2010·Cell and Tissue Research·Anna NeveFrancesco Paolo Cantatore
Jan 22, 2011·Nature Reviews. Molecular Cell Biology·César Nombela-ArrietaLeslie E Silberstein
May 2, 2012·Journal of Hematology & Oncology·Shihua WangRobert Chunhua Zhao
Jun 26, 2012·Journal of Bone and Mineral Research : the Official Journal of the American Society for Bone and Mineral Research·Lige SongJun Guo
Jul 19, 2013·The Korean Journal of Internal Medicine·Nayoun Kim, Seok-Goo Cho
Apr 18, 2014·Biomarkers in Cancer·Mohammed ShakerDominique Broccoli
Feb 1, 2015·Biochemical and Biophysical Research Communications·Peng DengQianming Chen
May 30, 2015·International Journal of Oral Science·Jing YangYing-Zi Yang
Jun 1, 2013·Bone Research·Brian E Grottkau, Yunfeng Lin

❮ Previous
Next ❯

Citations

Jun 15, 2016·Stem Cells International·Shoichiro KokabuEijiro Jimi
Sep 29, 2018·Cancer Science·Keishi YamashitaMasahiko Watanabe
Dec 29, 2017·Proceedings of the National Academy of Sciences of the United States of America·Xuefeng ZhaoJ Silvio Gutkind
Apr 26, 2017·International Journal of Experimental Pathology·Bibekananda SarkarAnil K Mantha

❮ Previous
Next ❯

Methods Mentioned

BETA
Reverse Transcription-PCR
PCR
electrophoresis
transfection

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.