Cysteine-functionalized polyaspartic acid: a polymer for coating and bioconjugation of nanoparticles and quantum dots

Langmuir : the ACS Journal of Surfaces and Colloids
Nikhil R JanaJackie Y Ying

Abstract

We have synthesized a biocompatible polyaspartic acid-based polymer (molecular weight approximately 15,000-25,000) with cysteine on its backbone for use as a capping ligand for functionalized Au, Ag, and CdSe@ZnS nanoparticles. Nearly monodisperse, hydrophobic Au and Ag nanoparticles and CdSe@ZnS quantum dots were first prepared in organic solvents via conventional synthesis and then ligand exchanged to derive polymer-coated water-soluble nanoparticles. Multiple thiol groups in the polymer backbone conferred excellent protection against aggregation of the nanoparticles, and the carboxylic acid groups in the polymer provided the possibility of covalent binding with antibodies. Compared to the conventional thiol-based ligands, this polymer coating led to superior colloidal stability under the experimental conditions involved in the bioconjugation and purification steps. Goat antihuman-IgG (anti-h-IgG) and antimouse epidermal growth factor receptor (anti-m-EGFR) antibodies were conjugated with the polymer-coated nanoparticles and successfully applied to protein detection. This polymer coating exhibited minimal nonspecific interaction with cells and could be broadly applied to cell labeling.

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Citations

Jun 9, 2011·Accounts of Chemical Research·Nandanan Erathodiyil, Jackie Y Ying
Mar 18, 2011·Langmuir : the ACS Journal of Surfaces and Colloids·Joshua ZylstraMathew M Maye
Sep 11, 2012·Langmuir : the ACS Journal of Surfaces and Colloids·Jun Sung Kang, T Andrew Taton
Oct 11, 2011·Advanced Drug Delivery Reviews·Hedi MattoussiHyon Bin Na
Mar 2, 2016·Langmuir : the ACS Journal of Surfaces and Colloids·Koushik DebnathNikhil R Jana
Dec 24, 2013·Biochimica Et Biophysica Acta·Mara WerwieHarald Paulsen
Dec 18, 2013·Chemical Communications : Chem Comm·Zhenzhen LiuLinyong Zhu
Feb 2, 2018·Molecules : a Journal of Synthetic Chemistry and Natural Product Chemistry·Stéphane W DuchironLuc Avérous
Feb 20, 2020·Journal of Materials Chemistry. B, Materials for Biology and Medicine·Prabhu Srinivas YavvariAasheesh Srivastava

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