PMID: 8584439Jan 1, 1996Paper

Cysteine-modifying reagents alter the gating of the rat cloned potassium channel Kv1.4

Pflügers Archiv : European journal of physiology
G J StephensB Robertson

Abstract

The effects of cysteine-modifying reagents on the gating of rat cloned Kv1.4 channels expressed in HEK-293 cells were examined using the whole-cell patch-clamp technique. Cells transfected with Kv1.4 expressed a rapidly inactivating K+ current with a mid-point of activation of -31 mV and a slope factor of 5 mV measured with tail current protocols in 35 mM Rb+ external solutions. The cysteine-specific oxidizing agents 2,2'-dithiobis-5-nitropyridine (DTBNP, 50 microM) and chloramine-T (CL-T, 500 microM) removed inactivation of Kv1.4. These effects were reversed by the reducing agent dithiothreitol (DTT, 10mM). In addition, DTBNP and CL-T also slowed Kv1.4 deactivation and increased the voltage sensitivity of deactivation. The action of cysteine-modifying reagents on Kv1.4 suggests that redox state affects channel gating, with oxidation tending to stabilize the open state of the channel, both by removing inactivation and slowing deactivation.

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Citations

Feb 27, 2001·The Journal of General Physiology·X D TangT Hoshi
Feb 24, 2004·Annual Review of Physiology·Xiang Dong TangToshinori Hoshi
Sep 2, 1997·Proceedings of the National Academy of Sciences of the United States of America·M A CiorbaT Hoshi
Apr 1, 2008·Biochemical and Biophysical Research Communications·Chi-Pan Hsieh
Apr 27, 2016·Aging and Disease·Federico Sesti
Feb 9, 2012·Biochimica Et Biophysica Acta·Nirakar SahooStefan H Heinemann
Dec 12, 2003·American Journal of Physiology. Cell Physiology·Daniel K MulkeyJay B Dean
May 12, 2004·The Journal of Biological Chemistry·Zhi-Hao ZhangQiang Wang
Feb 4, 2016·Circulation Research·David D GuttermanAndreas M Beyer
Jul 4, 2006·The Journal of Pharmacology and Experimental Therapeutics·Hye Sook AhnSang June Hahn
Jul 21, 2001·Molecular Endocrinology·P E MacDonaldM B Wheeler
Dec 10, 2003·Neuroreport·Bok Hee ChoiSang June Hahn

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