Cysteine oxidation and rundown of large-conductance Ca2+-dependent K+ channels

Biochemical and Biophysical Research Communications
Guangping ZhangT Hoshi

Abstract

Gating of Slo1 calcium- and voltage-gated potassium (BK) channels involves allosteric interactions among the channel pore, voltage sensors, and Ca(2+)-binding domains. The allosteric activation of the Slo1 channel is in turn modulated by a variety of regulatory processes, including oxidation. Cysteine oxidation alters functional properties of Slo1 channels and has been suggested to contribute to the decrease in the channel activity following patch excision often referred to as rundown. This study examined the biophysical mechanism of rundown and whether oxidation of cysteine residues located in the C-terminus of the human Slo1 channel (C430 and C911) plays a role. Comparison of the changes in activation properties in different concentrations of Ca(2+) among the wild-type, C430A, and C911A channels during rundown and by treatment with the oxidant hydrogen peroxide showed that oxidation of C430 and C911 markedly contributes to the rundown process.

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Citations

Sep 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Gonzalo BudelliLawrence Salkoff
Apr 29, 2011·The Journal of Biological Chemistry·Shin-Young RyuShey-Shing Sheu
Dec 17, 2010·Cardiovascular Research·Ursula Ravens, Erich Wettwer
Feb 7, 2009·Physiology·Shangwei HouToshinori Hoshi
Feb 22, 2014·Chemical Communications : Chem Comm·Stefan H HeinemannAlexander Schiller
Sep 18, 2013·Antioxidants & Redox Signaling·Nirakar SahooStefan H Heinemann
Mar 8, 2016·Brain Research·Rahul Patel, Federico Sesti
Apr 27, 2016·Aging and Disease·Federico Sesti
Apr 1, 2015·The Journal of General Physiology·Yutao TianToshinori Hoshi
Oct 24, 2009·Trends in Cell Biology·Federico SestiShi-Qing Cai
May 4, 2017·Antioxidants & Redox Signaling·Ashok KumarThomas C Foster
Jan 15, 2014·American Journal of Physiology. Lung Cellular and Molecular Physiology·Dahis ManzanaresMatthias Salathe
Jun 8, 2017·Antioxidants & Redox Signaling·Sue Goo RheeDongmin Kang
Dec 19, 2019·Frontiers in Molecular Neuroscience·Amber E PlanteAndrea L Meredith
Feb 6, 2018·Free Radical Biology & Medicine·Mia Y Bothwell, Martha U Gillette
Feb 12, 2021·Physiological Reviews·Rodrigo IturriagaVirend K Somers

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