Cysteine protease activated by expression of HIV-1 protease in transgenic mice. MIP26 (aquaporin-0) cleavage and cataract formation in vivo and ex vivo.

The Journal of Biological Chemistry
K P MittonP Russell

Abstract

Transgenic mice, homozygous for HIV-1 protease expression in the eye lens, display degradation of some lens crystallins and cytoskeletal proteins prior to cataract formation on postnatal days 23-25. Alterations to the internal lens hydration state also occur; therefore, the status of the aquaporin protein MIP26 was examined over postnatal days 16-25 to determine if it was altered during cataractogenesis. The MIP was identical in transgenic and control lenses until day 21. By postnatal day 25 (frank cataract), in the lenses obtained from transgenic animals, the 26-kDa band was absent and there was a concurrent increase in the proportion of MIP23. Immunoblotting demonstrated cleavage at the C terminus. Lenses were also maintained in an organ culture system to demonstrate that the cataractogenic process is inherent to the isolated lens and to determine the contribution of cysteine protease action. Organ culture experiments revealed a similar progression to nuclear cataract formation as seen in vivo. Two-dimensional gel analysis of the soluble lens crystallin fraction of organ cultured lenses revealed the same cleavage pattern as occurs in vivo. Organ culture of transgenic lenses with E64, a cysteine protease inhibitor, dramaticall...Continue Reading

References

May 14, 1979·Biochemical and Biophysical Research Communications·D Roy
Oct 1, 1992·AIDS·Y IwakuraH Shibuta
Dec 30, 1992·Biochemical and Biophysical Research Communications·M C ReddyP N Farnsworth
May 1, 1991·Trends in Biochemical Sciences·G J WistowA B Chepelinsky
Dec 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·G M Preston, P Agre
Sep 1, 1991·Current Eye Research·S J Girsch, C Peracchia
Apr 1, 1985·Current Eye Research·C Peracchia, S J Girsch
Dec 17, 1985·Biochemical and Biophysical Research Communications·C Peracchia, S J Girsch
Mar 28, 1986·Biochemical and Biophysical Research Communications·L Takemoto, M Takehana
Jan 1, 1985·The Journal of Membrane Biology·S J Girsch, C Peracchia
Aug 1, 1995·Current Opinion in Cell Biology·P AgreS Nielsen
Mar 1, 1994·Experimental Eye Research·S J TumminiaP Russell
Dec 20, 1995·Archives of Biochemistry and Biophysics·F A BettelheimP Russell

❮ Previous
Next ❯

Citations

May 14, 1998·Molecular Aspects of Medicine·A TaylorM Obin
Oct 29, 2008·Oncogene·B EwaldW Plunkett
Apr 27, 2001·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Y NakamuraT R Shearer
Nov 6, 2015·Pflügers Archiv : European journal of physiology·Kazuo Hosoi
Apr 29, 1998·American Journal of Human Genetics·J F Hejtmancik
Feb 13, 2001·Experimental Eye Research·S J TumminiaP Russell
Feb 21, 2014·The Journal of Biological Chemistry·Puttur SanthoshkumarK Krishna Sharma
Aug 15, 2002·The Journal of Biological Chemistry·R SenthilkumarK Krishna Sharma

❮ Previous
Next ❯

Related Concepts

Related Feeds

ASBMB Publications

The American Society for Biochemistry and Molecular Biology (ASBMB) includes the Journal of Biological Chemistry, Molecular & Cellular Proteomics, and the Journal of Lipid Research. Discover the latest research from ASBMB here.